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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 949 results for: org

Showing: 461 - 480 of 949 results

  1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA...therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional resources ...CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our CRISPR guide...

  2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038/s41593-019-0431-2  Neff, E.P. An AAV library...macaque, and man. Lab Anim 48, 264 (2019). https://doi.org/10.1038/s41684-019-0380-0...

  3. Lab Automation at Addgene

    Type
    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...Using Barcodes to Track Samples Resources at Addgene.org Find protocols for standard molecular biology...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519 More resources from ...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator... at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...CRISPR Michael Terns from of the University of Georgia studies the CRISPR/Cas system as it relates to ...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

  11. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...in the comments section below or contact blog@addgene.org if you'd like to write a more detailed post ...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...is “…where molecules become real”.  We sometimes forget that the models we create are around 30 million...reticulum. And graduate students, momentarily forgetting that the physical model is based on their own...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...

  13. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ... of your gene’s functional biology in a living organism. This is the first post in a three-part series...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ..., enter the common or scientific name into the Organism box. This small piece of information can significantly... of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from excitation...

  16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ... (e.g. Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to ...Contact our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for ...

  17. MXS Chaining

    Type
    Blog Post
    ...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...101: Green Fluorescent Protein Resources on Addgene.org Browse our Fluorescent Protein Collection Find...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...Worried about your budget? Email janeliafluor@janelia.hhmi.org to try out the JF dyes. Acknowledgements ...2015): 917-923. PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside...Fluorescent Protein Should I Use? Resources on Addgene.org Generate Fluorescent Protein Fusions Request...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...grandfather’s 100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep...home which happens very, very fast! I will never forget the time my eight-year-old daughter vehemently ...temple, my kids’ school, and with professional organizations supporting women in science and career development...
Showing: 461 - 480 of 949 results