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Showing: 461 - 480 of 961 results
  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post
    ...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...
  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post
    ...inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists to use ...optogenetics. Nat Methods 16:1176–1184 . https://doi.org/10.1038/s41592-019-0583-8 Additional resources ...chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene...
  3. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post
    ...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...
  4. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...
  5. Visualizing Protein Turnover In Situ

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    Blog Post
    ...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...
  6. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...
  7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post
    ...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...
  8. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ... antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019...Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101 Additional resources on the...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection...
  9. Transferable Skills Guide: Public Speaking

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    Blog Post
    ... Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly go over it now'?...Chinese undergrads. You get nervous and freeze, or forget a point, or talk extremely quickly. You realize...happens to other people, it’s normal and quickly forgotten, and when it happens to you, it’s noteworthy and...
  10. All in a Twist: dsRNA

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    Blog Post
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038/s41580-021-00430-1 More resources on the...
  11. Hot Plasmids and Viral Preps - March 2021

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    Blog Post
    ...accurately.   Priode and Haynes, protocols.io. 2021. dx.doi.org/10.17504/protocols.io.brgcm3sw     New system... cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for the...studies.    Duong et al., Genetics 2021. https://doi.org/10.1093/genetics/iyab006       The CRISPR corner...
  12. Hot Plasmids - October 2020

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    Blog Post
    ...   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a..., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting cell cycle...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR corner...
  13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA...therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional resources ...CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our CRISPR guide...
  14. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038/s41593-019-0431-2  Neff, E.P. An AAV library...macaque, and man. Lab Anim 48, 264 (2019). https://doi.org/10.1038/s41684-019-0380-0...
  15. Lab Automation at Addgene

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    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...Using Barcodes to Track Samples Resources at Addgene.org Find protocols for standard molecular biology...
  16. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519 More resources from ...
  17. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from...
  18. Plasmids 101: Terminators and PolyA signals

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    Blog Post
    ...English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator... at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...
  19. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...CRISPR Michael Terns from of the University of Georgia studies the CRISPR/Cas system as it relates to ...
  20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post
    ... of your gene’s functional biology in a living organism. This is the first post in a three-part series...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...
Showing: 461 - 480 of 961 results