We narrowed to 883 results for: prot

...target pathogenic proteins, as well as in the lab to deplete expression of proteins of interest. RNAi ..., I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome Res...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...

... for packaging, however, the isoforms of these proteins, as well as the viral LTRs differ. Therefore, ...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

...neurons and depending on the choice of channel protein, they can trigger neuronal excitation or inhibition... using recombinant adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...other plasmids in the deposit, NCBI sequence and protein data, as well as available backbone sequences. ...type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids pass...Educational Resources Sequence Analysis of a Plasmid Protocol ...

...specific to the type of module it is (Pol 2 protomer, Cas protein, ect...). Whatever individual components...approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus...

...programs can be put in place to provide community protection. Toehold switches for pathogen detection In ...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

...off-target editing than Cas9, confirming that this protein is well suited for genome editing.  Find Cpf1 Plasmids...100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of these options (2.7...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

...degradosome, which contains enzymes that cut RNA not protected by secondary structure. It is thought that poly...complex and involves two RNA polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting... tail is important for stability of the mRNA, protection from degradation, and is integral to the nuclear...

... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...Number of fragments assembled Golden Gate Assembly protocol* (Volume of 1mL Outgrowth plated) Correct assemblies...

...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range...selection by TnsD, a sequence-specific DNA binding protein. Selection removes donor bacteria and recipient...

... process, check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and...process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process...Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR...

...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014...

...are distributed under the terms of the UBMTA to protect our depositors’ IP, but there may also be extra...as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find additional... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...

...whole mouse brains expressing green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories... Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace...Tomer, L. Ye, B. Hsueh, K. Deisseroth. (2014) Nat Protoc., 9, 1682-1697. Pubmed. C. Soderblom, D.-H. Lee...

...error. Used in combination with standard barrier protocols, they can reduce the risk of accidental escape...research have conclusively shown that barrier protocols are vulnerable to human error. And with gene drives...original sequence, it can restore the original protein-coding sequence using a recoding strategy; a subsequent...

...vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters ...site, thereby resulting in co-expression of two proteins from a single mRNA. IRES was originally discovered...your gene of interest along with a fluorescent protein or selectable marker, it is easiest to start with...

...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...

...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...