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TypeBlog Post...Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;...
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Finding and Joining Your Dream Lab
TypeBlog Post...collaborations. Alternatively, perhaps the lab publishes and presents regularly with labs around the world... -
Hot Plasmids - February 2022
TypeBlog Post...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... the buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed ... -
Antibodies 101: Isotypes
TypeBlog Post...Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes that each have... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 blog posts Resources... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... from this collection in your experiments and published your data, please fill out this form and we’ll... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...survive, but thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...specific. Once inside the host cell, the virus unleashes its cargo – its own genome. Fig. 1:... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition... -
How Addgene Creates Content
TypeBlog Post... the example of our Antibody Guide, which was published alongside our new antibody services in the spring... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...of endogenous signaling activity in cells. Let’s shed some light on the key components of OptoNBs, how... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...