We narrowed to 534 results for: try

1. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive...

2. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...is. While communication styles in academia and industry vary drastically, he reassures that one can learn...

3. Plasmids 101: Yeast Vectors

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   ... multiple copies are required. yes   TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ... Stefan Hell, who won the 2014 Nobel Prize in Chemistry for his contributions to super-resolution light...

6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

7. Celebrating 15 Years of Scientific Sharing

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   ...request from us but also by high school students and industry scientists as a universal resource for information...

8. Antibodies 101: Affinity Reagents

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   ...Antigen Binding Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, ...

9. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...

10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...and suggestions, and even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive...

11. Developing Lab Management Software for Biology

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    Blog Post

    ...If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff. ...

12. Plasmids 101: Cre-lox

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    Blog Post

    ...-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives the loxP site directionality...

13. Working with Nuclear Receptors

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    Blog Post

    ...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...

14. Hot Plasmids - October 2020

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    ...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These...

16. Adenoviral Vector Production and Troubleshooting

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    ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston...

18. Corralling and Cornering the Goldilocks Antibody

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    ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

19. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...   Confirming the gene/insert by aligning to NCBI entry or using BLAST.   Confirming tags and fusion proteins...