We narrowed to 545 results for: SEC

1. CRISPR 101: Any Base Transversion Editors

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   .... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

2. Viral Production at Addgene

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   ...page for the corresponding catalog item (see maps section for images).     Figure 2: AAV Pro cells...

3. 5 Reasons to Use Reddit for Science Communication

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   ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...

4. Getting the Most from Your Lentiviral Transduction

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   ...successful lentiviral infection, happy virus is the second. When harvesting lentivirus, some labs prefer to...

5. Important Considerations in Optogenetics Behavioral Experiments

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   ...inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before you do ...

6. Plasmids 101: Control Plasmids

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   ...more below, but the papers cited in the reference section provide more in depth information. Technical replicates...

7. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ...intimidated by having to contact more senior colleagues. Secondly, science is highly technical and this has a natural...

8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...assigning team members to certain tasks, etc. How is security and user access to data defined within your lab...

9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...to find the right one Step 2 – Edit the cells a second time using a repair template that corrects the ...

10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...

11. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...Figure 3).  The Hehnly Lab used such a strategy to dissect the roles of three Rab proteins during a key stage...

12. Hot Biosensors 2022: Year-End Roundup

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    ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

13. CRISPR 101: Validating Your Genome Edit

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    ...helpful to know how many copies you are looking for. Secondly, categorize your edit – is it any frameshift mutation...

14. Hot Plasmids: Summer 2024

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    ...here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The staining patterns ...

15. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...fluorescent stain, you might be wondering which second measurement to choose for graphically displaying...

16. An Integrin Antibody Toolkit from IPI

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    ...that bind the same ligand, making it difficult to dissect the function of one integrin unless others are ...

17. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid...

18. Filming Science Videos in the Age of Social Distancing

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    ...their leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over...

19. Evolution of Lab Techniques

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    ...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...

20. Thoughts on the Future of Research 2015 Boston Symposium

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    ...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...