We narrowed to 556 results for: SEC

...associated with the library and reading their methods sections to see what worked.  Ready, optimize, transduce...

...(and therefore positive colonies) after each consecutive assembly and transformation round, due to the...

.... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

...duplicated during lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et...

...job, you’ll be in the best possible position to secure it! Further Reading and Outside Resources http...

...less technically challenging. Alongside there is a second-line team that takes on cases requiring in-depth...

...inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before you do ...

...more below, but the papers cited in the reference section provide more in depth information. Technical replicates...

...intimidated by having to contact more senior colleagues. Secondly, science is highly technical and this has a natural...

... Oftentimes, the water bath will have a spot to secure the thermometer in place. Turn the water bath on...placed inside. Water bath floats can be used to secure tubes in place and water bath weights can hold ...

...annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins in...activity of T5 Exonuclease and furthermore reduces secondary structure of ssDNA. (Rabe & Cepko, 2020). Incubate...

...assigning team members to certain tasks, etc. How is security and user access to data defined within your lab...

...dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...

...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...helpful to know how many copies you are looking for. Secondly, categorize your edit – is it any frameshift mutation...

...to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid...

...their leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over...

...to find the right one Step 2 – Edit the cells a second time using a repair template that corrects the ...

...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...

...that bind the same ligand, making it difficult to dissect the function of one integrin unless others are ...