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.... Transformation of bacteria with plasmids is important not only for studies in bacteria but also because...amplification. Higher efficiency cells are more important if you will be transforming with very small amounts...critical for Ampicillin resistance but is much more important for other antibiotic resistances. Plate some or... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

...light to detect small objects. Two of the most important properties of a microscope are magnification (...see several pieces of information, but the most important (for now) is the magnification power, such as ...be sure to carry it with two hands - one hand supporting the base and the other holding the arm - or use...your images in the future. Conclusion Like any important laboratory instrument, you should be sure to take...

...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

...EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ... reading. When the droplet reading is complete, export the data from all wells as a CSV file which will...

...desired insert. If you are in this situation, it is important to treat the digested vector backbone with a phosphatase...setting up the ligation reaction itself, it is important to determine the amount of cut insert and vector...being sure to increase the amount of buffer proportionally. 1μL of ligase should be sufficient for larger...

... be in the bottom portion of the gel, but all of the EtBr will be in the top portion and your bands will...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

...CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...of cell death upon antibiotic selection. It is important to monitor these cells regularly and replace the... the surviving cells in the culture, so it is important to do regular media changes and maintain optimal...

...vector cores. Our titers were similar to those reported by these institutions. This protocol is for a ...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...plasmids to find a suitable one. Some labs have reported better results when the plasmid is linearized....

...transduction efficiency by facilitating nuclear import of the vector’s preintegration complex in the transduced...below. Do not change the ends; these bases are important for cloning the oligos into the pLKO.1 TRC-cloning...temperature. This will take a few hours, but it is important for the cooling to occur slowly for the oligos...you have identified the positive clones, it is important to verify the insert by conducting a sequencing...

...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations...well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2...

...Background Information Bacterial glycerol stocks are important for long-term storage of plasmids. Although you...completely and will improve the shelf life. It is very important that you shake the glycerol before freezing (5...

...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

...represent the solids in your sample while the liquid portion is known as the supernatant. Depending on your ...supernatant, or both. Conclusion Centrifuges are important tools for many different protocols. Improper use...

...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

...access to IPI educational resources developed to support use of their antibody tools. Addgene also offers...

...series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers...

...connect with other researchers working with AAV, support open science, and maybe even win a $500 travel ...

...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

...and vote for your favorite! Our mascot who has supported us over the past eight years now has a new friend...

...Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors...

... students and teachers interested in JEI (the importance of word of mouth and personal networking!) and...

...working with Andrew, the head of our scientific support team, to tackle any experimental questions you ...

...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

...Conclusion Pipetting accurately and precisely is important since even small changes in the volume could affect...Beyond knowing the proper way to pipette, it’s important for scientists to calibrate their pipettes to ...

...experiences. It will contain all of the juicy and important details that you won’t find in the final publication...

...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...

...organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...

...background and education 3:49-4:35 : Kingdom on opportunities to go into science in Malawi 4:36-5:46 : How...