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We narrowed to 922 results for: Por

Showing: 721 - 750 of 922 results

  1. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...desired insert. If you are in this situation, it is important to treat the digested vector backbone with a phosphatase...setting up the ligation reaction itself, it is important to determine the amount of cut insert and vector...being sure to increase the amount of buffer proportionally. 1μL of ligase should be sufficient for larger...

  2. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... be in the bottom portion of the gel, but all of the EtBr will be in the top portion and your bands will...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...of cell death upon antibiotic selection. It is important to monitor these cells regularly and replace the... the surviving cells in the culture, so it is important to do regular media changes and maintain optimal...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...vector cores. Our titers were similar to those reported by these institutions. This protocol is for a ...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...plasmids to find a suitable one. Some labs have reported better results when the plasmid is linearized....

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...transduction efficiency by facilitating nuclear import of the vector’s preintegration complex in the transduced...below. Do not change the ends; these bases are important for cloning the oligos into the pLKO.1 TRC-cloning...temperature. This will take a few hours, but it is important for the cooling to occur slowly for the oligos...you have identified the positive clones, it is important to verify the insert by conducting a sequencing...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations...well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2...

  7. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Background Information Bacterial glycerol stocks are important for long-term storage of plasmids. Although you...completely and will improve the shelf life. It is very important that you shake the glycerol before freezing (5...

  8. Water Bath Protocol

    Type
    Protocol
    ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

  9. Centrifugation

    Type
    Protocol
    ...represent the solids in your sample while the liquid portion is known as the supernatant. Depending on your ...supernatant, or both. Conclusion Centrifuges are important tools for many different protocols. Improper use...

  10. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

  12. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers...

  13. Gibson Assembly Protocol

    Type
    Protocol
    ...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

  14. Immunocytochemistry

    Type
    Protocol
    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

  20. Addgene at SfN23!

    Type
    Blog Post
    ...working with Andrew, the head of our scientific support team, to tackle any experimental questions you ...

  21. Pipetting Protocol

    Type
    Protocol
    ...Conclusion Pipetting accurately and precisely is important since even small changes in the volume could affect...Beyond knowing the proper way to pipette, it’s important for scientists to calibrate their pipettes to ...

  25. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...

  29. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...They showed us their dedication to great customer support by dressing up as our Zendesk email system.  Our...

  30. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create this lovely...
Showing: 721 - 750 of 922 results