We narrowed to 1,029 results for: ELL

1. How Do I Place an Addgene Order Using a PO Number?

   Type

   Blog Post

   ...pricing information that is sent from a buyer to a seller. However, Addgene is a bit different from other...

2. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

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   Blog Post

   ...using a silica spin column. They can be pricey, but well worth it. 6. Edit your DNA sequence Finally, when...

3. Why Do I Need an MTA Anyway?

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   Blog Post

   ...are sharing their materials. Questions about intellectual property rights, pending publications, and commercial...

4. Reflections and Looking Ahead

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   Blog Post

   ...to open science and shared progress. But numbers tell only part of the story. This year, we made significant...

5. Bringing Sustainable Practices to the Lab: Innovations

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   ...waste management in our day-to-day lives, but not as well-integrated into our lab lives. Read on to learn ...

6. Scientist Networking: What is an Informational Interview?

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   Blog Post

   ...to develop relationships is before you need them (well really, always). Informational Interviews are for...

7. Top 10 Open Science Developments of 2013

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   Blog Post

   ...and content providers and participation from many well-respected and high-profile universities. We particularly...

8. IDP and your PI: A Roadmap for Career Planning and Personal Development

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   ...Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025...

9. Science as Art

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   Blog Post

   ...is now being appreciated on an aesthetic level as well. Beautiful microscope images which traditionally...

10. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    ...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

11. Who Gives a Tweet? 9 Facts About Scientists on Twitter

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    ...tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific...

12. New Norepinephrine Indicators: nLightG and nLightR

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    Blog Post

    ...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...

13. Future of Research Conference - Remarkable Opening Session

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    Blog Post

    ... institutions with allies engaged in training excellence to begin effecting change.  Panelists call for...

14. London Calling - Addgene’s New Europe Office in London

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    Blog Post

    ...anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools were pivotal in carrying out...

15. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    ...biotechnology products and biosensors for herbicides as well as developing yeast as source for dietary supplements...

16. An Addgene Summer Internship

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    Blog Post

    ...Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity training...

17. Build Your CRISPR Vocabulary

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    Blog Post

    ...nuclease, but there are many other Cas proteins as well, both natural and engineered. What these proteins...

18. We're Updating Our Hold for Publication Policy

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    Blog Post

    ...preprints and openly sharing materials are both excellent ways to provide transparency, support reproducibility...

19. Antibodies 101: Affinity Tags

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    Blog Post

    ...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...

20. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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    Blog Post

    ...categorized into a 3-level system on our website:   A yellow flame indicates that a plasmid has been requested...

21. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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    Blog Post

    ... or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left four...

22. Bringing Sustainable Practices to the Lab: Recycling

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    Blog Post

    ... should I do in the lab to help the environment? Well, you could: Collect the NEB and/or Millipore Sigma...

23. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    ...experiments to test nickase preferences using HEK293 cells. They started by comparing D10A and H840A total ...

24. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post

    ...Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation...

25. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

    ...efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321...

26. Plasmids 101: TOPO Cloning

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    ... Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this...

27. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ... of these more spatially confined experiments as well as those requiring reversibility that you cannot...

28. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    ... expression after AAV injection, what region and cell class ended up expressing your virus? If you were...

29. New and Upcoming Viral Vectors - December 2019

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    ... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

30. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ...journals. Since then, I developed a routine that works well for me to evaluate the submitted paper in addition...

31. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    Blog Post

    ...Despite Arabidopsis’ utility to science, and its well-known edible relatives, it is fair to say that most...

32. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post

    ..., and also a Professor of Molecular Genetics and Cell Biology at the University of Chicago. He was an ...

33. Trading Spaces - Visiting Scientist Edition

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    Blog Post

    ...mentor (if you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting...

34. Science Career Options

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    ...about it, but hobbies and volunteer experiences, as well as work history can really show that you have some...

35. 25 Work From Home Tips for Scientists

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    Blog Post

    ...routine and stick to it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try...

36. New and Upcoming Viral Vectors - September 2019

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    ...recommend validating the serotype in your target cells before conducting the experiment. Likewise, a lot...

37. New and Upcoming Viral Vectors - June 2019

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    ...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...

38. Components of CRISPR/Cas9

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    ...enzymes responsible for processing foreign DNA as well as the RNA guides required for endonuclease function...

39. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    ...s close to the right answer because if it’s not, well, your grade is lower.' ... When you are waking up...

40. Sweating the Small Stuff: Details in the Lab

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    ...out experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully...