We narrowed to 628 results for: PROC

1. Plasmids 101: FLEx Vectors

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   ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

2. Tips for Titering Your Lentiviral Preps

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   ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

3. The Materials Science of Optogenetics Experiments

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   ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...

4. How-to: 5 Steps to a Great Panel Discussion

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   ... Your audience may have a lot of real or self-proclaimed experts on the topic at hand. You can use this...

5. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ... thing into a marketable product. During this process, we had an idea that survived the literature review...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   ...indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment assemblies and over 90%...

7. 10 Basic tips for mammalian cell culture

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   ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...

8. Pooled Library Amplifications

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   ... reduce recombination during the amplification process. In liquid culture, all of the bacteria can grow...

9. Antibodies 101: Flow Compensation

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   ...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...

10. 3D Printing Meets CRISPR Cas9

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    ...protein. At some point during this interrogation process, they will almost invariably say something like...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...container before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after...

12. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...allowing them to optimize the compound production process. How have you used FP timers? Are you planning ...

13. Hot Plasmids: Winter 2025

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    ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

14. Hot Plasmids and Viral Preps - May 2021

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    ...could be used to study the role of aaRSs in these processes. To create the antibody toolbox, they screened...

15. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...types makes them useful models for developmental processes and promising therapeutic tools. The two unique...

16. Addgene's Cancer Collection Pages Connect the Research Community

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    ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...

17. Experimenting with New Careers while in Grad School

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    ... comfortable terms helped guide me through the process. As I mentioned, my graduate office staff was amazingly...

18. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...MMEJ doesn’t get a lot of press. However, this process accounts for a percentage of the mutations seen...

19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...that need to be considered during the planning process. For CRISPR experiments, the following framework...

20. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...timers for incubation steps, and save the entire process so you’ll have a record of your work. This feature...