We narrowed to 619 results for: PROC

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment assemblies and over 90%...

2. 10 Basic tips for mammalian cell culture

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   ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...container before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...allowing them to optimize the compound production process. How have you used FP timers? Are you planning ...

5. Hot Plasmids and Viral Preps - May 2021

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   ...could be used to study the role of aaRSs in these processes. To create the antibody toolbox, they screened...

6. Hot Plasmids: Winter 2025

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   ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

7. Pooled Library Amplifications

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   ... reduce recombination during the amplification process. In liquid culture, all of the bacteria can grow...

8. Antibodies 101: Flow Compensation

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   ...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...

9. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...protein. At some point during this interrogation process, they will almost invariably say something like...

10. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...

11. Experimenting with New Careers while in Grad School

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    ... comfortable terms helped guide me through the process. As I mentioned, my graduate office staff was amazingly...

12. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...MMEJ doesn’t get a lot of press. However, this process accounts for a percentage of the mutations seen...

13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...that need to be considered during the planning process. For CRISPR experiments, the following framework...

14. Plasmids 101: Multicistronic Vectors

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    ... a novel gene, is not always a straightforward process. Rather than try to directly detect your gene of...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...no PCR, no gel purification; the whole cloning process is so simplistic once you have your parts. That...

16. Isolating Genomic Regions of Interest with the CRISPR System

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    ... of a specific genomic region was an intensive process. One method required insertion of a recognition...

17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ... to genome editing of mouse zygotes, through a process known as CRISPR-EZ (CRISPR RNP Electroporation ...

18. Plasmids 101: Secondary Nanobody Toolbox

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    ...exclusively recognize only one primary antibody. This process can be avoided direct antibody fluorophore conjugates...

19. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...and train everyone on your team about that new process. You stepped up and got your team on the same page...

20. CRISPR 101: RNA Editing with Cas13

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    ...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...