...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...function of the distance between measured pixel and the soma. The initiation site identified in panel (C) is ...-independent versions that are trafficked to the soma via the Kv2.1 targeting motif as well as a pan-membrane...

...editing performance, we suggest that users select some of the best designs (e.g., top 5) and test them ...strengths of PRIDICT/PRIDICT2.0 while accounting for some of the inherent inaccuracies of computational prediction...Koeppel et al., 2023), their predictive power is sometimes limited across different targets due to variations...

...achieve. Most pooled library protocols aim for somewhere between 30x and 200x coverage, meaning that you...Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 144-188 bp...closer to your own pooled library. Be aware that some older libraries may not have amplification protocols...

...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...Although there are a few aspects of spectral flow that some users may find more difficult compared to conventional...

...fitness cost varied greatly between chromoproteins. In some cases, cells containing high-copy plasmid-borne ...though expressing the chromoproteins from the chromosome could get around this problem. There is no single...chromoproteins are and how they can be used, why not add some color into your research? If you have a favorite...

...yourself: I always find it odd when I walk up to someone’s poster, and then he/she dives right into the background... background without an introduction. If someone approaches your poster, introduce yourself and ask about...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

...may be transported through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've...software tools can be used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc...limitations and well-executed experiments require some consideration of FP properties. It is important ...

... cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...discovering and designing molecules that are showing some promise in their ability to inhibit Ras. Stephen...

...with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their favorite...research community if you've had success with it. Some great places to get your results out there include...advancing rapidly and it wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease...

...the protein's behavior in cells, in solution or something in between, you are using a tag with a serious..., 4]. Solutions to the polymer problem There are some simple solutions available for researchers to deal... equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers describing the...

...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good HDR systems, and NHEJ will...amplification. Given the inefficiency of HDR for knock-ins, some labs have used NHEJ for whole plasmid integration...

...! In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical...includes several different methods, so we've summarized some key features of common  delivery methods below: ... in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency of...

...computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns out over time...engineering qualities, what these parts allow is for someone to forward engineer a system of 10 genes. You can...can now forward engineer a 10-gene system to do something. We know from our testing of these parts that ...

...though exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful...shorter homology tracts have recently been used with some success (Yu et al., Nat Chem Biol). Mutate the ...HDR-promoted cycle longer. This approach may have some negative outcomes on viability if cells are arrested...

...has been a powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA ... template control (NTC) well is common. Here are some steps you can take to achieve a clean NTC: Have...is also being used in the detection of microbes. Some applications include measuring viable probiotics...

...or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita...types (Tatsis et al., 2007), potentially because some of the wild-type viruses can persist as a latent...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

...cell biologists talk to chemists? One reason is something called “the photon budget”: each sample has a ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing...where washing is difficult, such as intact tissue. Some chemical labeling strategies such as the bisarsenical...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...to analyze product size. This blog post discusses some of the key things to consider when performing colony...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

...versatile protein to suit their experimental needs, and some of the common applications in the lab. Why Green...instead related to the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...as the gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in ...

...questions from scientists all over the world looking for some additional information or clarification on these.... Frequently asked questions Now let's get into some of the nitty gritty and answer the questions those...designed specifically for transient expression (find some useful backbones here) or from viral transduction...