We narrowed to 878 results for: SOM

...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good HDR systems, and NHEJ will...amplification. Given the inefficiency of HDR for knock-ins, some labs have used NHEJ for whole plasmid integration...

...! In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical...includes several different methods, so we've summarized some key features of common  delivery methods below: ... in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency of...

...computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns out over time...engineering qualities, what these parts allow is for someone to forward engineer a system of 10 genes. You can...can now forward engineer a 10-gene system to do something. We know from our testing of these parts that ...

...though exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful...shorter homology tracts have recently been used with some success (Yu et al., Nat Chem Biol). Mutate the ...HDR-promoted cycle longer. This approach may have some negative outcomes on viability if cells are arrested...

...or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita...types (Tatsis et al., 2007), potentially because some of the wild-type viruses can persist as a latent...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

...cell biologists talk to chemists? One reason is something called “the photon budget”: each sample has a ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing...where washing is difficult, such as intact tissue. Some chemical labeling strategies such as the bisarsenical...

...has been a powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA ... template control (NTC) well is common. Here are some steps you can take to achieve a clean NTC: Have...is also being used in the detection of microbes. Some applications include measuring viable probiotics...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...to analyze product size. This blog post discusses some of the key things to consider when performing colony...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

...versatile protein to suit their experimental needs, and some of the common applications in the lab. Why Green...instead related to the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...as the gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in ...

...questions from scientists all over the world looking for some additional information or clarification on these.... Frequently asked questions Now let's get into some of the nitty gritty and answer the questions those...designed specifically for transient expression (find some useful backbones here) or from viral transduction...

...surface and a corresponding receptor in the host cell. Some of these envelope-receptor pairings are broad, allowing...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors... broad or specific to suit your needs. Check out some of these review articles to find tables and information...

...created a yeast-based directed evolution approach with some notable improvements. Directed evolution techniques...localization of biomolecules within cells. However, sometimes these fluorescent proteins might not localize ...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: New ...

...ricin uptake. The ricin screen did fail to identify some known ricin-regulators, but most of these genes ...show that genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such as non-specific...guides for off-target activity, Morgens et al drew some generalizable conclusions about off-target activity...

...use epitope tags to monitor single molecules - something researchers often want to do at neuronal synapses...and other FPs have the tendency to brightly label somata but not neuronal processes. In contrast, smFPs ...microscopy. Sample preparation for these techniques sometimes makes it more difficult to detect protein tags...

...evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides could be screened for their...microarrays and biochips. Ultimately, there are just some applications that lend themselves to aptamer-based...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...include the proper protein expression elements (ribosome recognition sequences, start codon, stop codons...attL2 only with attR2, and so on. Take a look at some of the Gateway Multisite plasmids available at Addgene...

...studying vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced... generating mutants. Unexpectedly, phenotypes sometimes differ depending on whether a gene is deactivated... using lineage tracing in zebrafish To highlight some of the features that make zebrafish useful, I’ll...

...transformation process could save the plant biologist some time… months, in fact. In the Lee-Parsons lab, ...Mortensen et al., 2019). Our method might provide some useful guidelines for the development of transient... different treatments on transformation success. Some reporter gene examples are below. The GUS gene ...

... the search to a ~4.5 Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA,...permissiveness While PHP.B has impressive CNS transduction in some strains of mice, these results are not reproduced...time AAV user tips from an AAV rookie  Learn about some considerations when using AAVs Learn more about ...

...worry, we’ve got you covered! Here you will find some useful tips to help you make your plasmid construction...tip, and arguably the most important, is to take some time to sit down, plan your cloning, and have a ...you make during the early stages of the process. Some questions to start with are: Is your sequence of...