We narrowed to 560 results for: cat.3

...school. The transition has been a bit rough for her (3 weeks in and there's a big office vs. lab culture ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...

...www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/... conferences are held every year in different locations all over the world. But simply attending the conference...work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great blog ...

...the membrane is cut so each lane is its own piece (3). After incubation, blocking, and washing, the membrane...protein concentration decreases).     Figure 3: Testing the antibody’s dynamic range. A) The protein...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins ...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...membrane into the psi membrane, that’s a good indication that the p membrane pores are too large.  Transfer...likely technical. White circles, for example, could indicate that you had air trapped between the membrane ...

...100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep a regular...other activities. Take care of yourself. Take a vacation alone with your partner – no kids. Get a massage...

... using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and...Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely...

...acceptable. Aims should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the...anticipated outcome, and payoff for each aim. Learn to communicate your story with only the most salient details...

...connectivity to other tools used in research; and (3) connectivity to open source data repositories. To... both accept deposit of data associated with publications into one of five general purpose open source...

...transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high transfection...transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence, harvest cells,...high levels of virus. HEK293T cells should be split 3 times a week: Monday: Plate 1x10 6 cells in a 75 cm...negative E. coli strain such as NEB stable. Dilute 1:3 (µg DNA:µg PEI) in 500 µL total of OptiPro SFM (per...of 1 mg/mL PEI (μL) 1:1 18.9 18.9 1:2 18.9 37.8 1:3 18.9 56.7 1:4 18.9 75.6 1:5 18.9 94.5 1:6 18.9 113.4...inhibit transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For...

...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus...SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9...jejuni (CJ) 3' NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW...the table above, 3' and 5' indicate on which end of targeted sequence the PAM is located. The majority of...continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches matters...double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides upstream of the PAM sequence. The...Each library typically contains ∼3–6 gRNAs per gene to ensure modification of every target gene, so CRISPR...

...total DNA to μg PEI ratios of 1:1, 1:2, 1:3 and 1:6. The 1:2 and 1:3 total DNA:PEI μg ratios provided high...post-transfection. Remove media, replace with fresh media. Day 3-4 (am): Harvest virus Equipment Class II, Type A2 ...obtaining high viral titer. 293T cells should be split 3 times a week: Monday: Plate 1×10 6 cells in a T75 ...enough PEI such that the ratio of μg DNA:μg PEI is 1:3 (1000 μL total per 10 cm dish). Using transfer plasmid...DNA μL of 1 mg/mL PEI 1:1 18.9 18.9 1:2 18.9 37.8 1:3 18.9 56.7 1:4 18.9 75.6 1:5 18.9 94.5 1:6 18.9 113.4...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45...

...pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection to yield...far, we’ve used the MUCICAT system in two ways: In our initial paper, we used MUCICAT to optimize the gene...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed...integration using CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems...providing users with a complete editing process. The MUCICAT system While the above systems are great for experiments...chromosomal integration (ex: genome editing), our MUCICAT system which is based on the CRISPR-associated ...unstable and lack precise control over copy number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC, pQCascade...

..." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput droplet...the other to guess a category by listing off things that fall into that category? Okay, let’s play. I’...’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet PCR...DNA dot ...concentration of viral genomes (by qPCR or other DNA quantification methods - see below), since each viral particle...value, physical titers don’t necessarily give any indication of the infectious titer. The infectious titer...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...optimized our assay in a few ways:  Absolute quantification by qPCR requires generating a standard curve...

...types can be found on Addgene’s website. Fig. 3: Cas9 repressors include dCas9 alone and a dCas9-KRAB... than ever to make precise, targeted genome modifications. Cas9 has been modified to enable researchers...instead.  Choosing a Cas9 for site-specific modifications Site-specific deletions and knockouts Some experiments...mutation at a specific site e.g., deleting the catalytic residue of a protein or knocking out a very small...flexibility as to where your sgRNA needs to be located and there may not be a NGG PAM site anywhere to... Beyond SpCas9.” Single base pair edits Is the location and type of edit you need to introduce highly ...Perhaps you need to introduce a single nucleotide modification at a defined amino acid to mimic a patient mutation...

... level should now be automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez...Labor’s general guide to the effect on higher education, you can see that those in teaching roles (such...frankly quite surprising. Consortia of higher education institutions submitted letters to the Department...centrally administered, which leads to many complications, including the inability of some institutions...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...of the postdoctoral workforce. Based on our communications with and research on these institutions, we...to the new level:  We are still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most...

... PMID: 14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous...gene at once. Therefore, applications that require simultaneous modification of all alleles of a gene,... recommends designing homology arms with the modification of interest, ordering them from a gene synthesis...with a homologous repair template. It is this application - the creation of sequence-specific knockins ...clones, making it unsuitable for most in vivo applications.  Third, even in the most optimized tissue-culture...tissue culture based approaches that require modification of only a single allele of a gene, such as the...efficiently deliver large repair templates for applications that require large gene fusions. The current...

... remember that antibodies recognize very specific 3-dimensional epitopes. Different sample processing ...knowing that where you see fluorescence indicates your target’s location. Some other terms you might see associated...immunofluorescence? Generally speaking, IF is an application in which antibodies that have been joined to ...bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness. Generating...distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), and will share...will share their findings either through their publications or as a review on a company website. It is important...Resources on the Addgene blog Learn about research applications of antibodies in Introduction to Antibodies ...

...Biotechniques 35.4 (2003). 812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination...positively charged polycation, polybrene. Interestingly, one study found that alternative polycations such as DEAE-dextran... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...lentivirus may want to consider testing a variety of cation and serum sources to ensure the best possible transduction...establishes the best conditions for specific applications, stick with them! Changes in such variables ...efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14...

...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking... gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated... sites can lead to excision, inversion, and translocation of DNA in a similar fashion as between loxP ...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus where they exert their recombinase...

...visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine deaminase base ...2019). TREE is a real-time, fluorescent based identification system for the isolation of base-edited cell...to BFP Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular...Harris RS (2018) A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9...

...activation. Second-generation plasmids include (Figure 3): Transfer plasmid — contains transgene and wild-type... expression from the LTR is Tat-dependent. Figure 3: Second-generation lentiviral plasmids. Created with... transfer plasmids also contain a deletion in the 3’ LTR to make them self-inactivating (SIN). This deletion...requirement for Tat. Always includes deletion in the 3' LTR for self-inactivation LTR Viral Promoter Wildtype...) plasmids. These plasmids have a deletion in the 3' LTR of the viral genome that is transferred into .... The inside out of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID...Molecular Therapy — Methods & Clinical Development , 3 , 16017. https://doi.org/10.1038/mtm.2016.17 PMID:...