We narrowed to 532 results for: lias

...your protein of interest (POI). This approach is reliable, but it’s overkill in many cases. Often, you may...

...fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen,...

...aptamers for imaging small non-coding RNAs in mammalian cells." Nature communications 9.1 (2018): 656....

...Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, J. C. (2010). “Random...

...from your single cells, as duplicates can’t be reliably analyzed on their staining signals. For this, ...

... Design-Build-Test-Learn cycles. If you’re not familiar with this, it’s a method for building and testing...

...of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles...

...You may be familiar with immunofluorescence (IF, often referred to as immunocytochemistry (ICC) when ...

...traditional tagging approaches insufficient. Vascular endothelial growth factor A (VEGF-A) is one of these challenging...

...for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...

...including belittling opinions, public professional humiliation, accusations regarding lack of effort, intimidating...

...display systems, such as bacterial, yeast, or mammalian display, allow for the presentation of your mutant...

...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...

...that steers the organization. As an Addgene vet familiar with the expertise and skills of many of my colleagues...

...Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering...

...limit of detection of ~5%. This means they do not reliably detect editing events that occur in less that ...

...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018...

...30°C and this is not ideal for experiments in mammalian cells. Dendra2 is another monomeric PA-FP which...

...she studies the development and regeneration of mammalian taste buds. Erin is also the President of Project...

... achieve this you could e.g. copy the list of “Aliases” for your gene from the Wikipedia page of the gene...