We narrowed to 883 results for: prot

...mutation, so you don’t accidentally change your protein of interest.   Fig. 2: Stop re-cutting ...further, you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase,...used to prime a Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9...

...mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to a repressive...MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root and Doench also ...2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification in gene...

...express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail ...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...-use AAV from Addgene here or check out these protocols if you want to package your own virus. ...

...that couldn’t package itself due to a few missing proteins. The envelope plasmid contained a viral envelope...for viral production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for...export of transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity...

...plasmids/viruses are needed to deliver all the protein and RNA components, and several sgRNAs must be ... start sites, all of which yield the same BDNF protein. To demonstrate the specificity that can be achieved...inserted human CD8, a gene that encodes a surface protein for which there are good flow cytometric antibodies...

...DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the nanopore...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...

...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell... Distinguishing correctly folded and misfolded proteins is essential for human health. Failure to do so...

...keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three...need share data with them including reports and protocols. To facilitate this, some ELNs allow you to invite...witnessing and audit trails. How does the ELN provider protect and respect your IP rights and data ownership? ...

... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...

...help prevent the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a...donor A may be capable of expressing a truncated protein. This can be avoided by excluding a stop codon ...AAV vectors with reduced expression of truncated proteins are safe and effective in the retina of a mouse...

...very minimum requires an Argonaute protein. This Argonaute protein is what binds the small RNA and either...cleaves the target mRNA or recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA...

...social contract citizens enter into in order to protect their rights (2). Less formally, governments are...for a given policy. In exchange, the government protects (and often funds [3]) scientists as well as other...Aeronautics and Space Agency, the Environmental Protection Agency, and the National Institutes of Health...

...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...many of the specifics - promoters used, precise protein sequences or domains, and methods of delivery -...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

...primarily by frameshift mutations in dystrophin, a protein essential for proper muscle function. Without functioning...are multiple common deletions that maintain the protein’s reading frame, leading to the production of a ...a smaller, but at least partially functional protein. Individuals with these mutations are often asymptomatic...

...bacterial strain that is deficient in recombinase protein RecA can reduce the frequency of multimerization...circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20... Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene blog:  Plasmids ...

... AAVs “unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and...receptor (AAVR)2   AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not strictly required.../α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR)2 Epidermal growth...

...Resources on Addgene.org Basic Molecular Biology Protocols Protocol Videos Molecular Biology Reference Pages  ... critical reagents for many molecular biology protocols, including vector-insert joining for recombinant...

...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps...

... pluripotent stem cells from urine samples. Nat Protoc, 2012. 7(12): p. 2080-9. PubMed PMID: 23138349....from human ES and patient-specific iPS cells. Nat Protoc, 2015. 10(7): p. 941-58. PubMed PMID: 26042384....cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p. 1836-46. PubMed PMID: 22976355...

...somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target efficacy is still ...but the good news is that you only need one. ProtospacerWB was developed with the purpose of applying CRISPR..., online), DESKGEN (commercial, online), or ProtospacerWB (academic, offline). While it remains to be ...