We narrowed to 724 results for: rel

...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share a...

... require careful selection or design to ensure reliable readout and avoid biases. The lab of Alejo Rodriguez-Fraticelli...cell membrane while AF, fused to a payload, is released into the synapse. Once AF binds to the postsynaptic...

...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8...

...serotype, as some rAAV serotypes are abundantly released into the culture media, while others remain cell-associated...Wilson, J. M. (2010). Efficient serotype-dependent release of functional vector into the culture medium during...

...individually count 500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency... anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the library, but generally needs...

...heartbeat – a muscle contraction – is directly related to the electrical impulse of the cardiac cells....and this voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It ...

...of compensation, which relies on user adjustment, spectral flow cytometry relies on “unmixing.” Unmixing...

...recombinantly append their proteins of interest with unrelated peptides that already had high quality antibodies...within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches” was not...

...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

...practices for assemblies of more than 10 modules often rely on two-step hierarchical approaches using different...pre-determined manner. If the original insert religates into its original vector, it will subsequently...

...volume. In many biological research situations, the relevant volume is that of the cell. For the physical behaviors...determine if an FP will dimerize in an experimentally relevant circumstance. For a more detailed discussion see...

...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...

...AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease in humans (and no vaccines...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...Designing donors for HDR The basis of the HDR pathway relies on repair from a template molecule, which is usually...

...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

... One key difference is the plasmid DNA must be released from the bacteria in order to serve as PCR template...reaction. You just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample...

...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

... important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted for their...explaining a gap in your job history or sharing relocation plans. They can be skills-based: if you're switching...

...works with Addgene in some way. For users, we just released a major redesign of how search works on our site...sprints is themed and the stories in that sprint relate to the theme. For example, we could have a shipping...

...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small...