... creating a robust data pipeline that would be reliable and available for our teams to use, one of our...container for its particular purpose and knew we could rely on the containers to produce the same output regardless...

...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

... important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted for their...explaining a gap in your job history or sharing relocation plans. They can be skills-based: if you're switching...

... so any results generated are reproducible and reliable. When you order a new cell line you will need ...conducted during projects to ensure the results are reliable. With this two-pronged approach, the many mistakes...

...literature to find all the applications of optogenetics relevant to your field. Ultimately, your question should... compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation...

...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

...a fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence...on collateral cleavage by the enzyme and instead relies on its activity on the target regions thus making...

...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...question? This should be something that anyone could relate to, that people would agree is important to understand...

...integration for their life cycle to continue. They rely on a viral protein called reverse transcriptase ...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells and...

... lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version...measurement of different RTKs. Although this sensor relies on a change in localization to the cell membrane...

...array of biological contexts.  Proximity labelling relies on the labelling of neighboring proteins within...interactions in vivo (Ge et al., 2019). This technique relies on an S. auerus transpeptidase sortase A variant...

... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

... traditional restriction enzyme cloning we once relied exclusively on. In this blog post, I will go over...isn’t ideal for synthetic biology standards, which rely up on the re-use of parts between experiments. In...

...etc. This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help... try to keep conversations focused on research relevant objectives and their tangible resources. For example...

...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...and test multiple mice with the drug rather than rely on the results of a single mouse. Biological replicates...

... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

...epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed repair (HDR) to...they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide...