We narrowed to 534 results for: try

1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   ...Regulation by Long Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146...

2. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   ...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature...

3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   ...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....

4. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   ...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...

5. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...

6. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...

7. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

8. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   ...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...

9. PhD Applications After COVID

   Type

   Blog Post

   ...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm...

10. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...

11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...

12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...

14. Tips for arabidopsis transformation

    Type

    Blog Post

    ...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...

15. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...

16. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

17. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    ...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...

19. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ...going to graduate school, Joe taught high school chemistry for a year. The teaching/mentoring bug never went...

20. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...