Working with Nuclear Receptors
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Blog Post
...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...
We narrowed to 540 results for: try
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CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...Regulation by Long Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post... Confirming the gene/insert by aligning to NCBI entry or using BLAST. Confirming tags and fusion proteins... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to... -
Hot Plasmids - October 2020
TypeBlog Post...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature... -
PhD Applications After COVID
TypeBlog Post...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc.... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...