We narrowed to 625 results for: Emb

1. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

2. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

3. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

4. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

5. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

8. Hot Plasmids - February 2022

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   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

9. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

10. Antibodies 101: Isotypes

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    Blog Post

    ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

11. Viral Vectors 101: Viral Applications

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    Blog Post

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

12. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

14. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

16. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

17. CRISPRainbow and Genome Visualization

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    Blog Post

    ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

18. Addgene: The First Twenty Years

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    Blog Post

    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

19. Summer SciComm: Preprints

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    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

20. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...