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We narrowed to 1,076 results for: TOR;

Showing: 751 - 780 of 1076 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...

  7. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...

  11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA sequences for your next....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

  14. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...., Langhorst, B. W., Bilotti, K., Pryor, J. M., Cantor, E. J., Canton, B., Knight, T. F., Thomas C. Evans...J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...? In this post we will review some of the main factors that affect isotype choice. Target and application...when choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety...

  16. Tips for Technical Support Calls

    Type
    Blog Post
    ...the experiments performed by our thousands of depositors. That means their original articles are your ...searches scientific articles, patents, and even some doctoral dissertations. Streamline the question asking...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  19. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

  21. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

  22. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...

  23. How to Write a Scientific Review Article

    Type
    Blog Post
    ...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  24. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...

  25. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...

  26. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  27. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen species, ...plasmids that liberate the targeting region from the vector. Alternatively, PCR-generated or commercially synthesized...

  29. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... a certain region or calculate a colocalization factor to assess the spatial relationship between the ...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...

  30. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...
Showing: 751 - 780 of 1076 results