We narrowed to 988 results for: igh

...approaches. Why researchers should have a mix of high-risk, high-reward and low-risk but reliable projects. ...

...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim...

...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ...

...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...

...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...

...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...

...precise genome edits, the recombinases used must have high activity and specificity with low cross-reactivity...recombinase/reporter pairs were inactive, even at very high recombinase levels. These data show that multiple...

...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...

...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation...

...growing open repository of CRISPR screen data that might be able to help you out. What is the Open Repository... cross-interrogation of different datasets that might otherwise be hard to identify. Figure 2: ...

...about science communication careers, you’re in the right place. For this series, I’ll interview three science... a writing position and looking for someone who might take over. I like writing and science, so I took...

...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

...celebrate our depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly... highly requested plasmids...

... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

...other reagents every day, and this crisis has highlighted the importance of access to quality materials...

... fused to mature fluorophores, and can bind and light up epitope tagged proteins in vivo. These probes...sites within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences...

...how aggressive my sentences might sound. What helps me in keeping the “right tone” is to address the comments...

...(Neuroscience AntiBody Open Resource). We are delighted to be partnered with Dr. James Trimmer of the ...this grant. Dr. Trimmer has created thousands of high-quality antibodies/nanobodies for neuroscience research...

...We hypothesized that this double stranding step might not actually be necessary. Since the AAV genome ...following DNA extraction, the [+] and [-] strands might naturally associate forming a double-stranded species...

...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ...

...focus on discussion and brainstorming Though, we might be forced to work from home and/or be in the lab...experience more interactive. The button is on the right of the Share Screen control panel on the top of ...

...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

... to address the effects of genetic variants in a high throughput manner. She helped develop a method that...shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere...

...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...