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  2. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

  3. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

  4. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  10. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  12. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  22. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  25. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

  26. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  27. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  28. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  29. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  30. How to Lead a Great Meeting

    Type
    Blog Post
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...
Showing: 751 - 780 of 980 results