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We narrowed to 993 results for: Des

Showing: 501 - 520 of 993 results

  1. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological atypicalities... Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes are everywhere and this ...shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...abstracts were driven by the quality of the study design or by their preconceived ideas about the controversial...transmission and how different fabrics and mask designs could work to slow the spread of illness. I later...Series: Masters of SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan... is also useful in a laboratory setting. When designing a plasmid to be inserted into a specific bacterium...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... craft sentences for abstracts and presentation slides, learn from audiences as they do science outreach...about whether the same principle applies to other slides in your deck.  If someone asks a question about...of a new science communication initiative that provides yet more scientists with opportunities to practice...

  7. Antibodies 101: ChIP

    Type
    Blog Post
    ...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works...up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...both tissue or cell type, amount of protein, and desired fragment length for your output assay. A longer...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...tables with fluorescent protein properties and provides a quick way to compare fluorescent proteins, e.g...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...practical brightness. Therefore, practical brightness provides a better picture of what can be expected in a ...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing....  Similar to a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in ...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...engineering approaches and scientific know-how to design/build/test their own project ideas using synthetic...newsletters, pairs teams with STEM mentors, and provides a nationally recognized platform in March to exhibit...s BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer...

  12. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ... also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges...wanted something that would allow automation. This design cuts out weeks of waiting and hands on labor. That...Addgene, now these can be available to anyone for a modest cost without having to start all over or write ...

  13. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...bacterial lac operon contains a gene called lacZ that encodes for the enzyme β-galactosidase. Expression of the...process is called α-complementation. The system described above was put to practical use in the following...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...and does not consider the missing or damaged nucleotides that are common to biological sources of DSBs...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate the ITR...interested in expressing multiple small genes, you can design single rAAV vectors with multiple genes packaged... an option (McCarty et al., 2001). This AAV is designed so that the single stranded DNA folds back on ...

  16. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...you labeled your gene of interest incorrectly on slides 8-16. Your notes disappear. The wrong version of... speaking is hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents... biomedical field, with very little text on the slides and notes instead of written speeches. When you...

  18. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...is tested following each immunization until the desired level of antibodies is reached - typically within...within 2-4 months. After the animal has the desired level of antibodies in its bloodstream, the antibodies...species. Pre-adsorbed polyclonal antibodies are designed for experiments where antibodies raised in several...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should be specific to that ortholog...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...genome editing. Such a system has been previously described for transcriptional activation/repression and ...

  20. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both... RNA toehold switch, a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream...components for gene disruption or CRISPRi. While designed to study the bee microbiome, these broad-host-...
Showing: 501 - 520 of 993 results