We narrowed to 706 results for: ESC;

1. Working with Nuclear Receptors

   Type

   Blog Post

   ...cardiovascular disease, cholesterol gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators...

2. How to Prepare for an Industry Interview

   Type

   Blog Post

   ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...

3. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   ... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

4. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   ...secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression...

6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

   ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

7. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   ..., can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into ...

8. Rabies and Neuronal Tracing

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   Blog Post

   ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   Blog Post

   ...partners other than those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify...

10. A Look at Addgene's QC Process

    Type

    Blog Post

    ...types of data for each of their plasmids, from descriptions of key components of the plasmids to partial...

11. Design Tips for Prime Editing

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    Blog Post

    ...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...

12. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable...

13. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...

14. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...Wen, B., Kraus, C., Miller, N. W., Steger, R. J., Escude Velasco, B., Bosch, J. A., Zirin, J. D., Viswanatha...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...the repertoire of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version...

16. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

17. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the...

18. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

19. Tips for Technical Support Calls

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    Blog Post

    ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

20. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

    ...driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws...