We narrowed to 637 results for: ESP

1. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   ...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   Blog Post

   ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....

3. Giving gRNAs a Facelift - Synthetic and Beyond

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   Blog Post

   ...triphosphate in the gRNA, reduce the innate immune response associated with gRNA introduction (Allen, et al...

4. Antibodies 101: Conjugation

   Type

   Blog Post

   ...cover all of the possible antibody conjugates (especially if you start to consider clinical applications...

5. Transferable Skills Guide: Public Speaking

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   Blog Post

   ...say the journal club was a rousing success–the response was a full sixty seconds of silence–but it was...

6. To Each HIS Own

   Type

   Blog Post

   ... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

7. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...Tetracycline-dependent promoters are engineered by placing tet response elements upstream of a minimal promoter. These...

8. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   ...from a vendor that you use frequently, with a responsive and knowledgeable technical support team, is ...

9. What's New in CRISPR - November 2021

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   Blog Post

   ... particles for delivery into mammalian cells, especially when fused to effector proteins for gene activation...

10. RNA Extraction Without A Kit

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    Blog Post

    ...Furthermore, RNases, or enzymes that degrade RNA, are especially hardy proteins that are found in and on everything...

11. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ... researchers wanting to consider sex is cost. Especially in animal research, they worry about needing ...

12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post

    ...bp gRNA target sequence and its 8-bp barcode, respectively. Once cloned into the storage vector, the pooled...

13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ... These systems, known as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more...

14. The Future of Research Symposium Boston 2015

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    Blog Post

    ...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...

15. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...and minuses, but Golden Gate cloning has been especially useful within both the synthetic biology and ...

16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ... shown me that I’m still in the dark ages with respect to my organization strategy. There are a number...

17. Working with Nuclear Receptors

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    Blog Post

    ...reproductive, developmental, metabolic, and immune response programs. NRs also encompass one of the most successful...

18. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...makes wide use of retroviral delivery systems, especially lentiviral vector-based CRISPR pooled libraries...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ... along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality. Since...

20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ... not all, S. pombe Met genes complement the corresponding S. cerevisiae met deficiencies (manuscript in...