We narrowed to 672 results for: FIE

1. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   ...particles have some advantages over the conventionally purified AAV vectors. They found that they have a higher...

2. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   ...from the malachite green (MG) dye, but has been modified with the addition of two iodine residues. Compared...

3. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   ... flies. Read the first post here. Once you’ve identified some fly stocks and other reagents of interest...

4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

   Type

   Blog Post

   ...Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated...

5. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ...from multiple samples will then also need to be quantified and re-normalized in order to be combined with...

6. The MTA at Addgene

   Type

   Blog Post

   ...store, and distribute your material under the specified terms. This acts as the signature on any subsequent...

7. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ...LinkedIn (or via industry newsletters such as FiercePharma), or sign up to be alerted about their new publications...

8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   ... has been a breakthrough for the genome-editing field. It has the possibility to treat human genetic diseases...

9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   ...contains a PDGFR transmembrane domain, followed by a modified form of the cytosolic C-terminal domain of Stargazin...

10. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    ...are single cell, eukaryotic organisms formally classified as fungi. They reproduce by either fission or...

11. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post

    ... they need to be edited to suit your particular field of research.   Citations tools DOI Citation Formatter...

12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    ... the gRNAs responsible for a phenotype can be identified by next-generation sequencing of the genomic ...

13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ... genome editing in human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6...

14. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    ... have the full plasmid sequence. Once you have purified plasmid DNA, this method can be done right in ...

15. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...resistance mutations, several new mutations were identified by targeting EvolvR to five dispersed regions...

16. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    ...mismatch assays require between 200 and 500 ng of purified PCR product. Low detection limits: Digital PCR...

17. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    ...plasmids in a ready-to-use viral format. Having identified this need in the community, we approached it ...

18. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    ...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ...

19. How Addgene Creates Content

    Type

    Blog Post

    ...resources with our antibody and content team, we identified what applications should be included and what...

20. AAV Q&A with Tim Miles

    Type

    Blog Post

    ...allow AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms...