We narrowed to 877 results for: HA

...specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%. Your experiment...Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization which...where only DNA sites bound by Cas are sequenced, has been tested with some success. The major con is that...

...localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity. All of...could be extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment ...engineering other enzymes. Conclusion We hope this post has illuminated some of the many tools available for ...

...personalized genetic medicine (Musunuru et al., 2025). Much has been made of the speed with which KJ’s successful...acceptable in every case, however, and recent work has already been published on improving base editing ...R. M., Pacesa, M., Smith, B. K., Christie, K. A., Ha, L. L., Meis, R. J., Clark, A. B., Spinner, A. D....

...goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most..._block %}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid..., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

... editors be close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at...or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with... abasic site. The same protein (or its homologs) has more than one name. Indel Insertion/deletion A ...

...with the macromolecules it is added to. Biotin also has an exposed side chain that can be easily manipulated...coated with avidin. The biotin-avidin interaction has also been adapted for use in immunohistochemistry... either the N or C terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing...

... beta-barrel structure. Although the beta barrel has no strong affinity for other proteins, it may still...modifications. Despite these potential issues, GFP has been used successfully in countless fusion proteins...localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization (Shaner et ...

...track single cell dynamics at the organism level has been made possible by tools that allow cells to become... such as neurons in the fly visual system, which has been difficult to study previously (Hadjieconomou...of cells in the intestinal crypt, and since then has become a powerful tool for understanding cell proliferation...

...neurons, directs high-level transgene expression, and has retrograde functionality to monitor and map neurons...doors in neuroscience. The retrograde serotype also has promising therapeutic applications; it can enhance...

...targeting, where siRNAs are ineffective and Cas13 has steep off-target costs. Additionally, this system...produced at Addgene and, as you might suspect, it has been one of our most commonly requested antibodies...

...sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems...

...slower, more traditional, QC process. While this has worked well for the early sets of SARS-CoV-2 materials...certain processes in both core operations. As it has been in much of the world, we have found ourselves...

...time Since the publication of our paper, our group has been inundated with requests to share our plasmids...). Also, we’ve got to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, ...

...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure...

... receiving training modules in synthetic biology has grown into a recognized name in the field of STEM...logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter and an ...

...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...

... chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety of promoters...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...means persuading an employer that your past work has prepared you to contribute at a high level to their...rewarding. A truly passionate community of scientists has formed around science communication, including the...

...science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often than not...these tools and to tell us how useful the service has been for their experiments. This helped reinforce...

...bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study ...