We narrowed to 856 results for: HA
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TypeBlog Post...time Since the publication of our paper, our group has been inundated with requests to share our plasmids...). Also, we’ve got to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, ...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post... size distribution and concentration of ApoB-LPs has been shown to have a strong correlation with cardiovascular...indicators difficult to work with. The Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in ... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often than not...these tools and to tell us how useful the service has been for their experiments. This helped reinforce... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...with the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...reagents. F1000Research, among many other journals, has joined a pilot project from the Neuroscience Information... up their own minds on whether the data analysis has been of good quality and whether they agree with ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...light of a different color instead. This approach has even been used in biosensors where BRET reports on... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging...components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning X gene, an ... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post... genes of interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate...rounds of replication are initiated before the cell has divided. This means that genes located near the origin... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...For example, the Levin lab from Tufts University has used Arch (green-light activated proton pump) and...Allen Discovery Center. The future of this field has a high potential for the advancement of research ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... Archon1 is bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds...levels are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...dye, but has been modified with the addition of two iodine residues. Compared to MG, MG-2I has increased... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...analytical ultracentrifugation yields an affinity and has been used to demonstrate that yellow fluorescent ...structure. Based on these data, we conclude that mKOk has dimerization tendency and is not suitable for protein... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...well or cannot be detected in a microscopy setup has zero practical brightness and is of no use. Therefore... practical brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...which is now easily available through Addgene and has already been requested dozens of times in 2 weeks...Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain, making... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... amounts or if the conjugated signaling molecule has a weak signal. For flow cytometry, secondary antibodies...introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal strength... -
Viral Vectors 101: Viral Applications
TypeBlog Post...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses... -
All in a Twist: dsRNA
TypeBlog Post...immune response machinery of the host. This pathway has been clinically exploited for antiviral purposes ...deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...