...2018 Microbe meeting. Soon after I arrived, I was looking through a paper-based, affordable, and portable...fascinated by the world that reveals itself when looking down a paper thin and very portable microscope....the scientist in everyone - everywhere! From ‘looking at’ to ‘listening to’ microbes But seeing microbes...

... expand reagent sharing even broader and began working with tech transfer offices to make reagents available...Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it...this year and along with it, wrote these tips for working with RNA and extracting RNA without a kit. 3. ...

...CRISPR For my editor’s choice picks this month, I’m picking two posts that describe methods to make single ...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as each one has something...

...this interest in the ways science is shared and in making it easier to share, we take special note as the...bump in citations for their trouble, even when all kinds of other factors, including journal impact factor...Way to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As...

...protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy... the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative...

...Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details the use of CRISPR...biallelic deletion clones:    Like the video? The Orkin and Bauer lab's related paper describes their methods...Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering...

...of lab BBQ parties - despite BBQs being wishful thinking in England. The Cepko Lab throws going away parties...celebrate each other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench... Dr. George Church (Harvard Medical School) for taking the time to speak with us throughout this series...

...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...use the search bar to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with... knowing the information is backed up and edit tracking will help you identify when others have added ...

...nLightR greatly expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues...Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods...Technique is Best for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene....

...because it finally feels to me like the postdocs are taking some steps to control their own future in science...speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our science training...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs...

...Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase overcomes ...forces acting on them. For the application we were looking at, magnetic isolation of circulating tumor cells..., highly stable and has this amazing chemistry locking the domain together - no one knows what for. Many...

...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate... Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, ... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications...

...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

...background noise. This is not terribly problematic when working with cell culture, because it is easy to harvest...harvest large quantities of cells. However, when working on specific tissues or organs in whole animal models...major technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to...

...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture...such as stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...

...Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration...

... cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving... a successful uptake of your DNA of interest by taking advantage of bacterial characteristics such as ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

...also involved in reinforcement learning, decision making, and motor control.  What do we understand about... 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection ...magnitudes of fluorescent responses. Studies on the kinetics of these sensors showed rapid response (60 or ...

...bunch. They don’t really know where I came from.” Working at eScience Labs, an educational company that creates...that provides scientific editing services. “I was working during the day and editing at night. It was not...moved out here, I was still editing from home and looking for a job.” But Caitlin’s persistence paid off:...

...headquarters. I decided to go ahead and apply; thinking I probably would not get selected for both the...compose and submit a manuscript that they were working on currently. Upon arrival to the headquarters,... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...