We narrowed to 771 results for: LIS
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TypeBlog Post... closing, these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...
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Working 9-5: an Addgene Co-op
TypeBlog Post...responsible for, in comparison to the other positions listed with a much less specific overview. It was immediately... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...may affect expected titers, a customer support specialist will reach out to you during the request stage... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post... come in the new year. I am proud of what we accomplished in 2023 and thrilled to be sharing our plans... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...the IBEX Knowledge-Base, with every contributor listed as an author. The IBEX Knowledge-Base is an... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...accessible practical guide which was recently published in Frontiers in Neuroanatomy. Here are the three... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...unique Addgene ID number, your publication will be listed on our website as a “Publication Citing this Plasmid... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post... of London and the University of California, specialising in Molecular Biology. She has worked in a range... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation... -
Plasmids 101: What is a plasmid?
TypeBlog Post...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that converts... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation.... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our Interview with Previous Awardee Kwabena ... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...], so making it a success feels like a real accomplishment.” He started working on them shortly after ... -
Hot Plasmids - October 2022
TypeBlog Post...!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin... -
Antibody Guide
TypeCollection...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated... -
Plasmids 101: Origin of Replication
TypeBlog Post...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...