We narrowed to 525 results for: MEA

1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   ...experiment and test them in parallel so you have a good measure of ICC assay specificity. We generally include ...

2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

   Type

   Blog Post

   ...environments. These studies provide quantitative measurements of cellular motility, interactions, and response...

3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

   Type

   Blog Post

   ...while the efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous...

4. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   ... makes choosing the right tool confusing and it means that there is no one-size-fits-all approach. You...

5. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   ...the construct is from our 'Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically...

6. CRISPR 101: Cytosine and Adenine Base Editors

   Type

   Blog Post

   ...important because ABEs have generally been slow, meaning that the Cas9 domain often lets go of the DNA before...

7. Using AAV for Neuronal Tracing

   Type

   Blog Post

   ...but overall with rather low efficiency. Not all measures of transport efficiency are consistent which may...

8. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ... in healthy humans. Many of the precautions are meant to protect individuals from potential accidents ...

9. Protocol - How to Perform Sequence Analysis

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   Protocol

   ...provider. My sequence has “N”s in it- what does that mean? “N” is the letter used to denote that the position...

10. Protocol - How to Inoculate a Bacterial Culture

    Type

    Protocol

    ...your specific protocol and conduct an OD600 to measure the density of your culture if needed. A good negative...

11. Ligation Independent Cloning

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    Protocol

    ...In our case, dGTP will be used in the reaction, meaning that T4 Pol will remove bases from the 3' end of...

12. Video Library

    Type

    Protocol

    ...while working. AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-associated virus...

13. Lab Safety for Biosafety Levels One and Two

    Type

    Protocol

    ...different types of hazards and to provide protective measures for each level, as each level has different safety...

14. Lentivirus Production

    Type

    Protocol

    ...ratios provided high transfection efficiencies as measured by the highest proportion of GFP positive cells...

15. Transfection for Recombinant Antibodies

    Type

    Protocol

    ...counting chamber on an automated cell counter and measure the live cell density and viability of the culture...

16. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...B7022S Procedure Pouring a Standard 1% Agarose Gel: Measure 1 g of agarose. Pro-Tip Agarose gels are commonly...

17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

    ...150 ng/μL to several μg/μL. The protocol below is meant to describe the general procedure for purifying ...

18. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...method results in a polyclonal cell population, meaning that the transgene has integrated in different ...

19. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...reference AAV of known titer, 100837-AAV1, and by measuring the titer of samples obtained from academic viral...

20. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ...with Coomassie blue, protein band intensities are measured using ImageJ software and a standard curve is ...