We narrowed to 847 results for: Met

...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

... Karl Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter ... using pharmacological or electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et...PMC2820367. Deisseroth, Karl. "Optogenetics." Nature methods 8.1 (2011): 26. PubMed PMID: 21191368. Deubner,...

...and volumes to determine the optimal injection parameters needed to transduce your cells of interest. Using...Once you have determined the optimal injection parameters for the serotype that best transduces your cells...performance in your system. It is very rare for a methods section to include all of the variables that need...

... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

...studying its structure can help us understand the parameters of CRISPR experimental design. Let’s dive into...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...

...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

...increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks...like to measure yours? More Reading Fenner M. "Altmetrics and Other Novel Measures for Scientific Impact...

...precipitation method or using a silica spin column available from several vendors. The precipitation method has ...

...of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science...

...CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...

... Cas systems, Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler ...they mined a database of microbial genome and metagenome from the Department of Energy’s Joint Genome ...

...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding...

... in terms of contamination, but consider flow cytometers. These fluidic machines handle biological samples...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...

...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...while some might argue that the only way to do something is to do it huge, there are times when a more ...

...this month, I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing...

... scientific research. Get an overview of these methods in this blog post. Hope you’ve enjoyed going through...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving...

...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...

... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...new x-ray technique provides scientist with a new method to explore the inner structures of delicate plants...

... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family...

... for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592...Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592...