10 Steps to a Perfect Science Talk
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Blog Post
... topics that are unfamiliar to you because the company you are visiting works on them. Of course, if some...
We narrowed to 538 results for: PAN
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Site Directed Mutagenesis by PCR
TypeBlog Post...In cases where the functionally important regions span several kb (or are GC-rich and therefore difficult... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post... master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...from academia altogether and instead started a company around his own research. He was clear on his science... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...of Bacterial Expression Systems. Check out our companion post describing protein expression strains, where... -
CRISPR 101: Off-Target Effects
TypeBlog Post... are less compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ... -
Plasmids 101: Repressible Promoters
TypeBlog Post...publication at Addgene. Riabinina, et al. “Improved and expanded Q-system reagents for genetic manipulations.” ... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute... -
Plasmids 101: Restriction Cloning
TypeBlog Post... digestion varies for different enzymes. Many companies now sell fast digest enzymes that can digest large... -
Important Considerations When Using AAVs
TypeBlog Post...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...transposon in vertebrates. It was discovered in Japanese medaka fish because its insertion into the fish... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...diversity of antibody reagents is continuing to expand, thanks to the efforts of antibody engineers. Many... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...assay kits are available from different biotech companies which are enzyme-based mycoplasma detection kits... -
Plan Your Experiment
TypeGuide...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...relative ease. Over the past decade, researchers have expanded the CRISPR toolbox to include many different types...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...their degradation within labelled cells limits the timespan available for tracking certain cell types. Another... -
Typing CRISPR Systems
TypeBlog Post..., Y., & Fu, Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...simply cease to exist. But then November hit and panic ensued as “alternative facts” became a part of our...