We narrowed to 537 results for: PAN

...In cases where the functionally important regions span several kb (or are GC-rich and therefore difficult...

... master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself...

...from academia altogether and instead started a company around his own research. He was clear on his science...

...of Bacterial Expression Systems. Check out our companion post describing protein expression strains, where...

...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...relative ease. Over the past decade, researchers have expanded the CRISPR toolbox to include many different types...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services...

...publication at Addgene. Riabinina, et al. “Improved and expanded Q-system reagents for genetic manipulations.” ...

...cell-surface shared proteome of astrocytes and neurons (CS SPAN) featured extracellular matrix proteins, cell adhesion...

... are less compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you...

...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ...

... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...

...piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute...

... digestion varies for different enzymes. Many companies now sell fast digest enzymes that can digest large...

...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 ...

...assay kits are available from different biotech companies which are enzyme-based mycoplasma detection kits...

...diversity of antibody reagents is continuing to expand, thanks to the efforts of antibody engineers. Many...

...transposon in vertebrates. It was discovered in Japanese medaka fish because its insertion into the fish...

...simply cease to exist. But then November hit and panic ensued as “alternative facts” became a part of our...

...their degradation within labelled cells limits the timespan available for tracking certain cell types. Another...

..., Y., & Fu, Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing...