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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 532 results for: PAN

Showing: 501 - 520 of 532 results

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... are less compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you...

  12. Typing CRISPR Systems

    Type
    Blog Post
    ..., Y., & Fu, Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...response over a broad dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...synaptic step, thus preventing further widespread expansion of RABV. This improvement resulted in a clearer...

  20. CRISPR Guide

    Type
    Guide
    ...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...., Strand, C., Joung, J. K., Kleinstiver, B. P., Pan, X., Huang, A., & Doench, J. G. (2020). Optimization...effects xCas9 3.7 - mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...
Showing: 501 - 520 of 532 results