Plasmids 101: Mammalian Vectors
Type
Blog Post
...your cells divide.
Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial...
We narrowed to 653 results for: PPL
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Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...can also allow multiplexing with experiments that supply both substrates and measure luciferase output at... -
How Addgene Creates Content
TypeBlog Post...antibody and content team, we identified what applications should be included and what basic information... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...require there to be a culture condition that can apply enough pressure to select for variants of interest... -
Antibodies 101: Isotypes
TypeBlog Post... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...Scientists can also use electroporation, the application of an electrical charge to cells, to increase... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... of research is important for downstream AAV applications. Tips & FAQs Due to their high GC content and... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ... -
Plasmids 101: Inducible Promoters
TypeBlog Post... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...