We narrowed to 537 results for: SHI

1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   Blog Post

   ...cellular aspects of CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested...

2. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   ...Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae, and Serratia marcescens...

3. Five Popular Model Organisms

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   Blog Post

   ... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...

5. Plasmids 101: Expressing shRNAs for RNA Interference

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   Blog Post

   ...used to refer to something that has fallen out of fashion but is still useful and most importantly, effective...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   ...PubMed Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman...

7. Antibodies 101: Beyond Surface Labeling

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   ...temperature, allowing for a flow analysis to be shifted to a later day. Note that intracellular labeling...

8. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ...    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single...

9. An Integrin Antibody Toolkit from IPI

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   Blog Post

   ...extended-open. When a ligand binds, it typically shifts the integrin from its closed to its open state,...

10. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...double-strand break repair: how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed...

11. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ...often leads to the generation of indels and thus frameshifts that disrupt the protein-coding capacity of a...

12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ...N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....

13. Viral Vectors 101: Optogenetic Tools

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    Blog Post

    ...C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K...

14. CRISPR 101: Off-Target Effects

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    Blog Post

    ...double strand break (DSB), the event required for frameshift and knock-in mutations. If one nickase acts on...

15. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent...

16. Finding Your Perfect Job After University

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    Blog Post

    ...would find a genetics related role soon after finishing my MSc. The masters program was 6 months of classes...

17. A History of Genome Engineering in Popular Culture

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    Blog Post

    ...were space and robots. Warping to stars in space ships and the rise of killer machines that exterminate...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...manager at genOway.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

19. Important Considerations When Using AAVs

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    Blog Post

    ...gene delivery in an efficient and non-pathogenic fashion. Work improving AAV tissue tropism, transduction...

20. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ..., which I’d say is pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting...