...allowing novice researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more...

...both brain function and dysfunction. First, let’s start with the basics: What is optogenetics? Optogenetics...

...which microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic...

... gRNAs that target your list of selected genes. Start by choosing which “Reference Library” you’d like...

...interpret flow cytometry data users will typically start by creating a dot plot of FSC versus SSC that allows...

...markers with any fluorophore. Bleeding Before we start building your first panel, we'll talk about one ...

...reviewed in [Yan & Bruchez, 2015]). To get you started, two robust orthogonal labeling strategies are ...

...When you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!... eBook Collection When you're not sure where to start or need a handy reference, Addgene's eBooks are ...

...construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing situation: you only...

...Conclusion Finally, we can summarize the process from start to finish:   Figure 9: Cartoon summary of...

...This may be due to speed, cost, availability of starting materials or just personal preference. Check out... fragments you want to assemble before you get started. Check out Addgene's website for easy to use MoClo...

...plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific plasmids ...

...in protein expression. (If you're just getting started, check out our Introduction to PCR.) But if you're...

...various applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics...Overview of CRISPRi/a Targeting dCas9 to transcription start sites is sufficient to repress transcription by ...recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading the single-stranded DNA, unraveling DNA...

...mechanisms. Rare observations like these may be a starting point for future developments in the field of ...

...replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were...

... found at Addgene, so what are you waiting for? Start building up your own designer bacteria. St-Pierre...

...Addgene has created the following guides to help you start learning about various scientific concepts related...

...provirus. 3' LTR in cis Terminates transcription started by 5' LTR by the addition of a poly A tract just...

...or RSV. 3' LTR in cis Terminates transcription started by 5' LTR by the addition of a poly A tract just...