We narrowed to 570 results for: Star;

...doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper...

...weaknesses of the options. Use this blog as a guide. Start a document to record notes and observations so you...

...research enterprise time and funds. It’s time to start putting them into practice!   Additional Resources...

...both brain function and dysfunction. First, let’s start with the basics: What is optogenetics? Optogenetics...

...allowing novice researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more...

...well. Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant...

...dsDNA-dependent exonuclease.  Exo will degrade linear dsDNA starting from the 5’ end and generate 2 possible products...

...markers with any fluorophore. Bleeding Before we start building your first panel, we'll talk about one ...

...interpret flow cytometry data users will typically start by creating a dot plot of FSC versus SSC that allows...

...which microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic...

... gRNAs that target your list of selected genes. Start by choosing which “Reference Library” you’d like...

...reviewed in [Yan & Bruchez, 2015]). To get you started, two robust orthogonal labeling strategies are ...

...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...understanding of your experimental goal, you are ready to start designing the specific components (Cas enzyme and...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...

... GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative... that binds to complementary DNA and acts as a starting point for the DNA polymerase. Thus to replicate...each amino acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource...Alternative start codons include CUG in eukaryotes and GUG in prokaryotes. Common Epitope Tags Tag Amino...

...construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing situation: you only...

...Conclusion Finally, we can summarize the process from start to finish:   Figure 9: Cartoon summary of...

...in protein expression. (If you're just getting started, check out our Introduction to PCR.) But if you're...

...plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific plasmids ...

...mechanisms. Rare observations like these may be a starting point for future developments in the field of ...

...replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were...