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We narrowed to 958 results for: Tes

Showing: 501 - 520 of 958 results

  1. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...for scientific titles generated from the United States Standard Occupational Classification System  and...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...scientists was Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists...

  2. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...to make it more accessible. Other accessibility updates include a printer-friendly design and alt text ...on all images. We have also made accessibility updates to our website, which receives 3.8 million visitors...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

  3. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally...homepage While users are increasingly accessing websites other than through the ‘front door,’ the homepage...Additionally we also wanted to highlight our blog, daily updates, and offer better exploration throughout the site...

  4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... may appear to be binding at particular genomic sites when in fact they are just non-specific or indirectly... MNase. Once MNase has been localized to target sites, the nuclease is briefly activated to digest the... an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets,...

  6. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...used in optogenetic studies are not native to vertebrates and are commonly introduced in zebrafish through... a neural circuit involved in locomotion that generates periodic motor commands for rhythmic movements...  Find plasmids from the Isacoff lab! They then tested what kind of swimming behavior, spontaneous swim...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression...bacterial cells and selectively grown on antibiotic plates.  Importantly, because the bacteria from which ...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...breakthroughs often occur when the unexpected infiltrates the realm of focused exploration. Here, we delve...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...beneficial to carry a notebook at all times (or use the notes app on your phone) to capture fleeting thoughts ...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here... The sense of trust and comfort I had with my teammates acted as my safety net and trampoline all at once... I really believe that the culture at Addgene cultivates trust between coworkers and that trust then translated...

  10. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became...mutations require individualized optimizations, safety testing, and approval. In contrast, a system that can deliver... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...opsonization of IgG and IgM antibodies on a pathogen activates the immune system's classical complement pathway.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

  12. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky...time to completely digest the template DNA. Note—templates must have been isolated from methylation competent...

  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in ...collection of empty backbones that have been designed, tested, and published by academic scientists. To help ...a mutated strain or a mutated version of YGOI to test the different phenotypes of the specific mutations...

  14. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... can’t safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase...I got good at this despite the fact that my lab mates thought this was hilarious.  Birth of the baby gel...

  15. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...labs have websites listing alumi and it is easy to use publication records, university websites, ResearchGate... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

  16. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...Avidin and its derivatives have four biotin-binding sites per molecule so, theoretically, saturated avidin...were unable to cleave TEV within the protein we tested, it may be possible with other fusion proteins ...bacteria significantly enhance protein folding (tested using real science). Make a stock solution of 2.5...

  17. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...in a competitive transplant assay in the mouse intestine, demonstrating its utility in long-term tracking...

  18. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and transduction...host of other AAV serotypes; it will be exciting to test this approach with different capsids. Most importantly...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences...molecular pathways, I found myself curious about other routes – In terms of both types and topics of work. A ...rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination to be ...

  20. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...building that someone from a different company had tested positive for COVID-19 (it turned out to be an unfounded...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...
Showing: 501 - 520 of 958 results