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...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...

...others in the field where to find it. A good way to reach others in the science blogosphere is to read and... great place to find opportunities for science outreach. Here in the North Carolina Research Triangle ...He got a little money for his trouble, too. 7)   Reach out to science writers in your area or at your institution...

...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder, Melina...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

...unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether...recombinant antibodies via Addgene to maximize the reach and impact of your work. Please email [email protected] scientists to easily share plasmids with each other. Together, we can transform the antibody sharing...

...RecBCD in bacteria. When two repeating elements love each other very much undergo recombination, two smaller...plasmids and testing your prep can save you the headache of troubleshooting failed experiments. Pick the...References and resources References Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (...

...focus on the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The DNA...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...

... need to dive as deep into your research at an outreach event as you might in a scientific paper. Build...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

...Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository...really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic Biology Engineering... database. Through a series of simulations and machine learning, they created a classifier that predicts...

...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors...PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE...

...generation of various flux imbalances. The conventional approach has typically been to either systematically delete...networks and multiplex gene control. However, these approaches are often met with the challenges of regulatory... be layered with distinct programs controlled by each dCas9 regulatory switch. By no means limited to ...

... but also how to fix it when things go awry. To reach that goal, his group develops tools to tinker with...within them work together or perhaps compete against each other. Getting two-color stimulation has been a ...time? Boyden: There are always considerations for each species. This could be a three or four hour conversation...

...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...Assess your interest in a different field Outreach activities (e.g. Volunteer at a local high school...

...millions, if not billions, of dollars, but by approaching it from a new direction, we hope to do it much...Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is to discover key human proteins that can be ...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

...basically, instead of having to sign individual MTAs for each request, the depositing organization signs one form...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...  Rachel Leeson...

...Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral...looking to try out a new technique or reagent! Racheal Komuhendo is a Lab Technician at Addgene who is... Racheal Komuhendo...

... found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus, genes...making a new fly with hedgehog under a new promoter each time a new hypothesis is tested. Controlling multiple...roughly half as much protein as overexpression of each gene individually. Applications of the Gal4/UAS ...

...bibliographic databases available, Web of Science. For each publication, Web of Science provides basic bibliographic... lends weight to the argument that Addgene is achieving its goal of making plasmid-based science more ...Special thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this...

...microscopy) on the material pages associated with each item. There are multiple CRISPR tools available ...and David Root as ready-to-use lentiviral preps. Each library uses nearly 80,000 gRNAs to target around...infection and at least one screening-scale experiment achieving the gRNA representation recommended in the publications...