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We narrowed to 819 results for: cell

Showing: 501 - 520 of 819 results

  1. RUBY-Red Siliques

    Type
    Blog Post
    ...graduate student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology...untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...Fluorescence Activated Cell Sorting (FACS), allowing for enrichment of successfully targeted cells that can be used...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun,...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...school at MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse...genetic screen to identify the factors that regulate cell division. And that really got me hooked on genetics...high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier especially finds...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...Expression of firefly luciferase from a cell-free mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE...not secreted, so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence...expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly... viral infection efficiency, to study changes in cell physiology, or as a reference level of expression...Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...

  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean...Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

  6. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...Mammalian cells provide scientists with the means to study biological processes on the cellular level instead...with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly ...

  7. A Control for All Seasons

    Type
    Blog Post
    ...to detect dead or dying cells so that you can base your analysis on living cells only (for example, propidium...This tends to be most problematic when staining cells or tissues that are the same species as your primary...propidium iodide only stains dead cells). Compensation controls address the spectral overlap that occurs...Quantitative Fluorescence Microscopy.” Journal of Cell Biology, vol. 185, no. 7, Rockefeller University...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...two-photon microscopy. Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags...Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389/fcell.2022.880107. KRaION potassium...sensor relies on a change in localization to the cell membrane and not a direct change in the fluorescent...of receptor tyrosine kinase signaling in living cells. bioRxiv 2022.08.13.503850; doi: 10.1101/2022.08.13.503850...better-matched to the potassium concentrations in cells than existing sensors. Using genome mining and structure-guided...fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent...inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors....

  9. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...). The Heroes of CRISPR. Cell, 164(1), 18–28. https://doi.org/10.1016/j.cell.2015.12.041  Musunuru, K....birth. Within a seven-month whirlwind of developing cell and mouse model systems, testing base editing variants...reporting genome editing by CRISPR in mammalian cells appeared in 2013 (Cong et al., 2013; reviewed in... The first ABE was about 50% efficient in human cells on average, but its effectiveness varied depending... the chromosomal location, sequence context, and cell type. Multiple labs worked to improve its effectiveness...would be tested for their base-editing efficacy in cellular models of KJ’s CPS1 mutation. Every genetic target...base editors that could target KJ’s mutation in cellular models tested both the SpG and SpRY variants of...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... edit A → Y (Y = T or C) in 67.17% of screening cells (Tong, Wang, et al., 2023). Based on this promising...Cas9 nicks the non-edited strand to encourage the cell to repair the DNA using the edited strand as a template... efficient A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650...programmable T-to-G and T-to-C base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j....efficient T-to-G and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https:...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The...

  11. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...use electro-competent cells instead of the more common chemically-competent cells. You should perform, ... manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-...1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total...colonies, you might want to use higher competency cells. Additionally, if your final product is going to...

  12. Lentivirus Plasmids

    Type
    Collection
    ...Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. These viruses... in the viral particle) is delivered to a target cell upon infection. When lentivirus is used for research...the researcher wants delivered to specific target cells. This genome is encoded by plasmids called "transfer...encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce viral...

  13. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...high light doses to the cells. Thin static samples Ex: Fixed monolayers of cells or thin (<15 μm) tissue...1). Thin dynamic samples Ex: monolayers of live cells with fluorescently labelled, motile structures These... 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos >15 μm Thsee are some...Total internal reflection fluorescence microscopy in cell biology. Methods Enzymol 361, 1-33 (2003). PubMed...Richardson DS, Lichtman JW. Clarifying Tissue Clearing. Cell 162, 246-257 (2015). PubMed PMID: 26186186.  Additional...Biological Imaging and a Lecturer on Molecular and Cellular Biology at Harvard University. No matter whether...Biological Imaging and a Lecturer on Molecular and Cellular Biology at Harvard University.     Additional ...

  14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... in mammalian cells Hu et al. compared xCas9 activity with wt SpCas9 in mammalian cells, finding xCas9...NG and GAT PAMs. xCas9 3.7 Activity in Mammalian Cells Construct NGG PAM NGA PAM NGC PAM NGT... percent editing efficiencies (edit frequency in cell population measured with NGS) are shown as percentages...

  15. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... make it difficult to properly gate your desired cell population. By compensating between fluorescent ... your fluorescent markers are staining for. If a cell is positive for both markers, it will show up as...Figure 3: Flow plot for a double positive sample. The cell population in the top right corner of the plot is...learning! Ashlyn has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research...

  16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... acid (DAP), a component of the cell wall. Once in the recipient cell, the CRISPRi DNA (purple) flanked... is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt gene function...mobilize these plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi...

  17. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in...be used for screening transcription factors for cellular programming or cancer drug resistance. Depositor...transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...

  18. Worm Expression Resources

    Type
    Collection
    ...Worms are particularly well suited for studies in cell biology, development, and aging. Scientists can .... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...AAV can transduce both dividing and non-dividing cells with a low immune response and low toxicity. Although...

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...reliably detect HMOs. Fatima’s first design is a whole cell biosensor with two components - an enzyme that breaks...binding results in an increase in fluorescence of cells that actively produce the HMOs. Fatima has demonstrated...out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional Resources on the Addgene...Awardees: Xin Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their...proposes using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria...
Showing: 501 - 520 of 819 results