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We narrowed to 704 results for: des.1

Showing: 501 - 520 of 704 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics... Roth BL (2015) DREADDs (Designer Receptors Exclusively Activated by Designer Drugs): Chemogenetic Tools...less invasive and more flexible as it relies on designer ligands that can be simply introduced by injection...lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements...should be carefully controlled for in experimental design.   Optogenetics Chemogenetics Timing Fast ...Find our chemogenetics and optogenetics science guides ...

  2. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...information Since the inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand...infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met at a lindy...to possess the correct phages will test. These includes labs with a large number of different phages or...

  3. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle M., et al...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...

  4. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR Genome Editing...given our CRISPR Plasmids and Resources a fresh new design, sorting the plasmids we have available by category...capabilities. Epigenetics: CRISPR has made it possible to design programmable epigenome editing tools, and the field...cleavage in bacteria. In mammalian systems, Cas13a degrades only the specified RNA target. Bonus: It displays...

  5. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...  Figure 1: Overview of the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-... sequences in the CRISPR array that will become guides for future targeted degradation. Before integration...biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources...

  6. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...in less than a minute (1). Green Listed is a new software tool used to design gRNAs for custom CRISPR...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR... massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...Institutet, in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000...moment, 13 full genome SpCas9 knockout libraries designed by different academic labs, targeting mouse, human...drug targets  Another interesting approach is to design screens against known druggable targets, preferentially...library from Addgene, and then use Green Listed to design a smaller validation screen using selected gRNAs...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...that work for our experiments!)     Figure 1: The IBEX Imaging Community front page. That’s...spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors... And the dataset is rich. Experimental data includes reagent type, target species, target name, host... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

  9. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... everyone was very approachable.     Fig. 1: Examples of DNA sequencing QC work    In addition...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...it relates to other teams at the company. This provides new (and old) employees with information on how...

  10. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...datasets by computational biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...briefly describe some tips for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your...typically writes the grants that fund your research, guides the research directions of the lab, gives you constructive...Thesis committee members provide expertise in designing and interpreting experiments, as well as career... there a tractable question or are you simply describing a phenomenon? Exploration can be important, but...presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation to a...story I can tell with my data?  Can I make better slides that more effectively tell a story? Generally,...or functional models or more helpful background slides. If you don't have much new data, speculate on ...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... antibody recognizes and binds to.   Figure 1: Labeled diagram of an antibody including Fc, Fab,...45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...antibody, which recognizes the primary antibody and provides the method of detection. You’ll also want to use...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, ...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...Figure 1: Map of a plasmid with its elements described below.   Vector Element Description Origin...molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid. A plasmid is a...why each is important. Our “Plasmids 101” series designed to educate all levels of scientists and plasmid...basics and spend more time developing cleverly designed experiments and innovative solutions necessary...utilized in the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally...the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose...

  14. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...into three main effector functions.  Figure 1: Fc effector functions in an immune response to a ...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally... 2022). So the next time you see an antibody description with the Fc domain as only providing structure...

  15. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...help you decide if a master’s might be for you. 1. Are you interested in master’s-level jobs? Ultimately...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...and industry, often means moving from the lab to a desk, although there’s often technical career tracks ...

  16. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...vast collection of empty backbones that have been designed, tested, and published by academic scientists....find the vector that fits your experiments, I've described below some of the most frequently requested vectors...protein with a specific resin (e.g. 6xHis containing pDEST-HisMBP). After purification the protein can be released...vectors. Consider your applications and experimental design. You can create a new strain with a disrupted gene...

  18. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells ... whose expression varies depending on transgene design and delivery. They generated hPSC lines with AAVS1...

  19. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ... email in a busy inbox. Updated policy As of July 1, 2025, we are updating our Hold for Publication policy... release plasmids on hold if they are clearly described in a journal publication. Plasmids will be released...

  20. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... serotonin, and much more. Check them out! Table 1: New ready-to-use viral vectors in the Addgene repository...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...
Showing: 501 - 520 of 704 results