Addgene

We narrowed to 625 results for: gats

Showing: 501 - 520 of 625 results

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Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...
Early Career Researcher Toolbox: Finding Relevant Papers

Type

Blog Post

... if you have any favorite tips and tricks for navigating the always growing sea of scientific literature...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

...RNA, which occurs in three phases: initiation, elongation, and termination. In a previous post we discussed...
The Materials Science of Optogenetics Experiments

Type

Blog Post

...activation/inactivation kinetics. For example, the light-gated cation channel, Channelrhodopsin 2 (ChR2), exists...
How-to: 5 Steps to a Great Panel Discussion

Type

Blog Post

...maybe 5) and no more. Too many panelists will negatively affect timing and the liveliness of the discussion...
10 Basic tips for mammalian cell culture

Type

Blog Post

...A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...expression makes it an invaluable tool in the interrogation of gene function. However, there are limitations...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...the amilCP and tsPurple chromoproteins to create negative selection markers for allelic exchange. These ...
Magnetic Control of Proteins: More than a Dream

Type

Blog Post

...Robinson, J. T. (2019). Magnetic Entropy as a Proposed Gating Mechanism for Magnetogenetic Ion Channels. Biophysical...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...Addgene’s free educational resources help scientists navigate that learning curve. As of 2023, our CRISPR guide...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

... temperature Taq polymerase can synthesize and elongate the target DNA quickly and accurately. ...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...within a multi-tRNA synthetase complex, the team investigated whether the generated antibodies could bind ...
Of Myc and Men

Type

Blog Post

...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...affected by the plasmid size, nature of the insert, propagation strain, growth conditions, and the material used...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

... immunizing reversal drive at the same time to mitigate the potential effects of an accidental release3...
Plasmids 101: Multicistronic Vectors

Type

Blog Post

...cell antigen receptor-CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Holst et ...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...HDR-promoted cycle longer. This approach may have some negative outcomes on viability if cells are arrested too...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

...References Germline variation contributes to false negatives in CRISPR-based experiments with varying burden...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...membranes and these detergents avoid some of the negative effects caused by organic solvents. However, detergents...

Showing: 501 - 520 of 625 results

Results per page:

Hot Plasmids and Viral Preps - January 2021

Early Career Researcher Toolbox: Finding Relevant Papers

Plasmids 101: Terminators and PolyA signals

The Materials Science of Optogenetics Experiments

How-to: 5 Steps to a Great Panel Discussion

10 Basic tips for mammalian cell culture

Truncated gRNAs for Regulating Gene Expression

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Magnetic Control of Proteins: More than a Dream

Twenty Years of Addgene Sharing: CRISPR

Polymerase Chain Reaction Overview and Applications

Hot Plasmids and Viral Preps - May 2021

Of Myc and Men

Cloning Mammalian Cells with the Agarose Method

Plasmids 101: Optimizing Plasmid Yields

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Plasmids 101: Multicistronic Vectors

CRISPR 101: Making a Knock-In Cell Line

Uncovering Ancestral Bias in CRISPR Research

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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