We narrowed to 1,082 results for: ica;

...1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic inheritance factors. He...hereditary determinant”. His proposal, however, was basically ignored. A separate term, “episome”, defined as... plasmids have greatly impacted many areas of biological research and have been key in advancing our knowledge... as bacterial conjugation and recombination, replication and topology, and cloning and gene expression...National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed PMID: 24043817...Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535...PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial Plasmids In Vitro.” Proceedings...

...for Neurobiology has been working on genetically encoded indicators of calcium and other small molecules...of interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...his team is now applying it to improve upon an indicator they developed and published in 2010 for visualizing...tools useful, including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind...

...humanistic and philosophical implications. As an example, a group at Caltech used an optical fiber together...handle on some of these mysteries. Another application is clinical; can we find ways to treat patients? Over...Optogenetics is a toolset that lets you turn the electrical activity of neurons on or off using pulses of...photosynthetic or photosensory molecules that come from basically every branch on the tree of life. Our most recent...implant already. If we can use light to more specifically activate a set of neurons, maybe we could treat...Addgene Alerts box. References: Independent Optical Excitation of Distinct Neural Populations. Nathan...

... regions of homology at their ends, which are typically created by PCR. Then, the fragments are incubated...neighboring fragments can be created via PCR amplification with primers that contain the appropriate homologous... tools that help you design primers for PCR amplification of fragments to incorporate such regions of ...competent cells. The success rate of this reaction is typically fairly high, so there is usually no need to screen...assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those where the vector...CRISPR technology and is described in a recent publication by the Lockey lab (Wang, et al. 2015). In this...the bacterial chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a...

...improve our shipment processes. After reading a publication describing how short term storage on dry ice ...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...exposed to CO2/dry ice. All samples were tested in duplicate. What we found Not all tubes (or rather, not ... the samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting... at -80°C. We then thawed the tubes after the indicated incubation periods, and measured the pH. We found...

...Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent protein...one of the most indispensable tools in modern biological research.  Any microscopist will tell you that...non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...maturation, oligomerization), many of which are dramatically affected by environmental conditions (such as...decision remains spread across literature in publications that introduce these tools. Years ago, Kurt...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy ...

...population (typically cells or small organisms) and then assaying for a functional or phenotypical outcome... daunting task of finding new factors in your biological system of interest, the so-called “fishing expedition...that both attempt to identify new players in a biological process. Players can be proteins, genes, enzymes...obligatory growth advantage – that is, you are not specifically looking at cells that can survive. Instead, ..., the entirety of the small molecule realm is typically not feasible to test in any given screening experiment...selection process. Non-functional traits will be automatically eliminated, unlike in screens. Selections begin...the answer that much faster? In reality, many biological questions are not conducive to selections and...

...comparable in efficacy to dCas12a systems in both gene activation and base editing applications.  Figure...stability in cells, and use it in a variety of applications. This set of new anti-FLAG frankebodies includes...to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling various targets... and anti-HA frankenbodies have a variety of applications, including live-cell imaging, single-molecule...members or students new to protein expression and purification. Figure 3: The Penn State Ladder run ...challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas system ...: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021.   Created...

...transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does...pages containing lists of plasmids from the indicated publications. You can request all of these plasmids ...to think about DNA in this context, but also specifically study it. They might be researching enhancer...--directly or indirectly--many other areas of biological research. If you’re looking for resources that...you study how chromatin effects your favorite biological phenomenon, there’s a reasonable good chance ...below pull up lists of plasmids containing the indicated gene, and you can subscribe to these pages to ...RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl ...

...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d ...RspCas13d. RspWYL1 also increased EsCas13d activity, indicating that WYL domain-containing proteins may be broader...even when guides were tiled across a target RNA, indicating that this enzyme does not cleave at a predictable...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...target essentially any RNA in a cell. Since catalytically dead dCasRx maintains its RNA-binding properties...enhancers. After AAV-mediated delivery to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved...

...their idea is. While communication styles in academia and industry vary drastically, he reassures that ... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...fit for this set of technologies, effectively communicating the advantages embodied, and driving the enterprise...He explained that entrepreneurs must clearly communicate their idea at various levels of complexity and...biggest challenge during this time was learning to communicate effectively to non-scientific audiences. "There...for the company; this will help avoid future complications and potential conflicts that waste money and...

...communities aim to significantly improve the reproducibility and reliability of science publications. Peer reviewing...should I choose? Writing a research paper for publication in a scientific journal requires several considerations...Information Framework (NIF) called the Resource Identification Initiative. During this project, authors are...likely that researchers have enough information to replicate your experiments, making research more reproducible...happens before the manuscript is accepted for publication and is, in most cases, blind (meaning you don...revised before it can be published, this can delay publication and manuscripts may go through several rounds...a novel form of peer review that occurs post-publication and is completely open; the reviewer’s name and...

...variety of research applications, including making transgenic cell lines and genetically-modified cell lines...facilitates the integration of the transposon specifically at ‘TTAA’ sites randomly dispersed in the genome...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...of interest. The piggyBac™ system allows for significantly enhanced random integration efficiencies that... If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates...of the piggyBac™  transposase/transposon gene modification system and the scarless, Footprint-Free™ removal...re-integration from the piggyBac™ donor, which is typically found with the wild-type piggyBac™ transposase...

...expression by various mechanisms. The RNA guide is typically 20 nts and the genomic locus must often be proximal... to keep in mind, but likely will not have a significant impact on your data. You are trying to generate...product with CRISPR to (hopefully) be used in human clinical trials. An off-target rate of 5% is far too high...Cas-OFFinder, CCTop, and many more. While such software typically only considers DNA sequence and doesn’t take into...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...selectively sequenced to determine if there are significant editing events, serving as a proxy for total ...For a candidate site-specific approach, mRNA quantification or western blot of putative off-targets would...

...Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control of neural activity. Nature Neuroscience...Ganguli, S., & Deisseroth, K. (2019). Cortical layer–specific critical dynamics triggering perception. Science... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...through multiple intra-cellular signaling cascades. Optical methods for controlling GPCR activation have been...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

...    Red calcium indicators PinkyCaMP and FRCaMPi By Mike Lacy Many popular genetically encoded calcium...cell mitochondrial transfer tracker Red calcium indicators PinkyCaMP and FRCaMPi Micropeptide killswitch...Workflow for cell-surface protein labeling and identification. Reproduced from Wu et al. (2025) under CC ...calcium indicators (GECIs) are available, but red GECIs have been limited compared to other colors. Two recent...their sensors in a variety of neuronal imaging applications, including confocal and widefield imaging, two-photon...sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at...any kind of condensate. The killswitch can be genetically fused to a protein of interest or targeted to...

...pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded biosensors and iPSC-derived...Scientific Advisor. It’s possible that solute channels typically expressed on other cell types can compensate for.... “This proof of concept study will help in identification of new targets that could greatly enhance our...understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...Terry Hébert, a professor and assistant dean of biomedical science education at McGill University.  These...58 individuals with GNB1 disorder since the identification of this disease in 2016. The lab aims to “expand...

...since other signaling networks can also be non-specifically activated and, like the game of telephone, often...must be transmitted by intermediate messengers. Optical control of kinase activity can provide greater ...greater spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist...Problem:  There is no common method for creating optically controlled kinases. The Solution: In Zhou et al...and MEK1 is active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates...

... antibodies may be indicated on occasion, usually after other signal amplification techniques have been...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...For the latter three, secondary antibodies are indicated when the protein of interest is found in low amounts...been ruled out.  Ease of access Signal amplification isn’t the only benefit of secondary antibodies. Finding...which means that indirect methods are often significantly longer than direct ones.  Even though the secondary...quantitative analysis of a sample. Instead, relative quantification can be done using either the direct or the ...

...from the serum. Protein A/G purification Protein A or Protein G purification is one common method. These... the desired level of antibodies is reached - typically within 2-4 months. After the animal has the desired...reagent that works for your species. Affinity purification Only up to 5% of the IgG antibodies produced...specific for the immunogen. A different type of purification can be performed to isolate antibodies only ...inexpensive. Their short production time and the bulk purification steps keep the cost low compared to other antibody...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...