We narrowed to 534 results for: invs

1. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   ...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   ...homology directed repair, these methods do not involve double strand break (DSB) formation and do not ...

3. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   ...environment itself. At Addgene we hope that all parties involved in making academic research a successful and enjoyable...

4. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   ...signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms that are ...

5. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   ...factors to consider. First, the titer required is inversely related to transduction efficacy: the higher the...

6. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   ...during our quality control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which...

7. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   ...through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19...

8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   ...then the result of their recombination is the inversion of the DNA between them (Fig. 1A below). In the...

9. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...

10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...base of former mentees, we encourage them to get involved and give back by becoming mentors to new students...

11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    ...remains exposed for a longer time. The aerosols are invisible to naked eyes and hence cannot be detected until...

12. Antibodies 101: Validation

    Type

    Blog Post

    ...- it is hard to distinguish between specific, invariable expression and non-specific, background signal...

13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual colonies...

14. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    ... non-scientists alike, need to stay active and involved if we wish to make positive change. Below I present...

15. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    ...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

16. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    ...model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    ...purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ...

18. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    ...were able to extravasate from the bloodstream to invade lymph nodes and scan antigen presenting cells, ...

19. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    ...combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID: 25751063...

20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ... cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors- ...