We narrowed to 565 results for: mme

1. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   ...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

2. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   ... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

3. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...

4. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   ...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

5. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   ...localizes to unintended sites and performs its programmed function there, this is an off-target effect....

6. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

7. Hot Plasmids: Spring 2025

   Type

   Blog Post

   ...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   ...system. As we cannot make universal serotype recommendations, the following table focuses on tissue tropism...

9. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

10. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    ...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

12. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    ...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    ...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

14. Finding Your Science Policy Path

    Type

    Blog Post

    ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

15. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...

16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

18. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    ...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology...

19. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    ... glass and the water-like buffer the sample is immersed in. Although the excitation light is completely...

20. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    ... effector function. Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic...