We narrowed to 560 results for: mme

1. Viral Vectors 101: Optogenetic Tools

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   ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

2. Plasmids 101: Repressible Promoters

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   ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

3. Antibodies 101: The Basics of Western Blotting

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   ...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

4. Hot Plasmids: Spring 2025

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   ...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

5. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

6. Finding Your Science Policy Path

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   ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

7. A History of Genome Engineering in Popular Culture

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   ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...

8. Quick Guide to All Things Lentivirus

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   ...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

9. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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   ...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

11. Sequencing Options for CRISPR Genotyping

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    ...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology...

12. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

14. Plasmids 101: Modular Cloning Applications and Kits

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    ...   Figure 1: A chart with MoClo kit recommendations for different modal organisms and applications...

15. Cancer and the Immune System: Deciphering the Relationship

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    ... effector function. Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic...

16. Antibodies 101: Flow Cytometry

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    ...arising from the secondary antibody.  It is also recommended that users include a viability control as this...

17. Antibodies 101: Introduction to Antibodies

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    ... antibodies for neuroscience research from the Trimmer lab ...

18. Typing CRISPR Systems

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    ... C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

19. Which Fluorescence Microscopy Techniques is Best for Me?

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    ... glass and the water-like buffer the sample is immersed in. Although the excitation light is completely...

20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...easily isolated somatic cells that have been reprogrammed to iPSCs successfully [10-12]. The next stage...