We narrowed to 568 results for: mme

1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   ...with all plasmids in the repository, we highly recommend reading the associated publications or protocols...

2. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   ... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...

3. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   ...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial...

4. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   ...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

5. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   ... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

6. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...

7. Hot Plasmids: Spring 2025

   Type

   Blog Post

   ...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   .... Though we cannot make universal serotype recommendations, the following table summarizes tissue tropism...

9. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   ...localizes to unintended sites and performs its programmed function there, this is an off-target effect....

10. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

11. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

12. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    ...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

14. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    ...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    ...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

16. Finding Your Science Policy Path

    Type

    Blog Post

    ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

18. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    ...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

20. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...