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We narrowed to 760 results for: mpo

Showing: 501 - 520 of 760 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...excitation and emission wavelengths, it is also important to know the brightness of individual fluorophores...antibody-conjugated labeling. Even though the two compounds are completely different in nature (eGFP being...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...through click chemistry. Although these multi-component systems can be complicated to implement, they ...Ulrichs, H., Gaska, I., & Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by ...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...cell’s size, granularity, and target protein composition. It is used for a variety of applications such...electronics systems, FACS instruments have specialized components that can divert a subset of cells. During FACS...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...fragments, allowing ordered assembly of multiple components into a destination vector. The first step in ...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...
Showing: 501 - 520 of 760 results