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...the CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes respectively:...

... the biomedical research community. Along these lines, Darrell recently joined the Addgene Board of Directors... various lung diseases. Chapter 10: How Darrel defines Open Science and how he practices and promotes ...open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity. Chapter...

...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...in line with current Web Content Accessibility Guidelines (WCAG). These changes mean our content is both... Chonnettia Jones...

...culture of engagement in the issues at hand and eagerness to get, as well as give, information to make improvements...to the manager. Team members rarely rush to give honest feedback to the manager for a variety of reasons... frequency. Anonymity can be a great way to get honest feedback, but shouldn't be the only way.   From...

... cell’s surface proteins and this interaction determines the specificity and efficiency of the AAV serotype...each approach and to follow best practices and guidelines. It was wonderful to see the development of this...by the workshop participants  - a testament to openness and sharing in science.  We’re excited to support...

...perform pooled CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific...Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis of human metastasis cohorts...knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1 Double...

... more readily enable research. Often “normal” timelines that could be weeks to months were reduced to ...Institute                                       Chinese Academy of Medical Sciences University of Texas...Stanford University University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership...

...Plasmids can carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic...

... comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder standard...

...and can deliver large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas...systems; Cas12k in Type V-K systems; Cascade shown) combines with a donor template and transposon proteins ...Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines prime editing with a site-specific recombinase...

...able to obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis... and I were unsure if there were any successful clones because the bands were all in the wrong place. ...

... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

...Targeted manipulation Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends...precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and chemogenetic tools need...region Impacts all cells expressing receptors Invasiveness Mice require permanent intracranial implant ...

...easy to spot meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant...specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic...changing it from a puzzle to decrypt to a tool to be harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's...

...it takes to be a good scientist are not the same ones it takes to be a competent manager (much less a ...strong sense of engagement in the work Behind-the-scenes support from their bosses and employers. Availability...may not necessarily be comfortable with imposed deadlines (there’s that need for flexibility, sometimes ...

...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express...At Addgene, we have a vast collection of empty backbones that have been designed, tested, and published...vector). Check out our complete guide to empty backbones for additional details and guidance for choosing...

...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...visualization or isolation, then using only a few lanes or a small portion of the gel.  What a waste?  I... pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less ...

...knockout screening, sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent their expression...expression. The knockout cells can be used to identify genes that underlie a particular phenotype. Figure...knockout screens: sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9...

... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ... be picked and sequenced for potential positive clones. Once you introduce your 3’-end “A” overhang insert...

...sometimes impossible) timelines—others are usually waiting Work is concentrated to business hours (mostly), ...with peer mentors, to discuss your strengths, weaknesses, interests and passions is another great way ...