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We narrowed to 985 results for: nin

Showing: 501 - 520 of 985 results

  1. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...characteristic absorbance spectra, which you can detect by examining the A260/280 and A260/230 ratios.  In common practice...you are purifying plasmid DNA from bacteria or cleaning up a PCR product, a common material you are trying...complementary assay, such as a fluorescence based assay or running an agarose gel. In the case of plasmids, the use...allow, you might be better off starting from the beginning. Before trying again, revisit your purification...

  2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...search results: More than 200 different GCaMP containing plasmids are available for distribution. If ...already created and deposited it with Addgene. Combining search terms with filters lets you drill down ...) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify the source...bacterial resistance marker used to grow plasmid containing bacterial cultures Bacterial Resistance: Use ...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    ...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...insertions (usually via seed growth on antibiotic-containing media) A similar process occurs in nature; ...plants should show no signs of stress, such as anthocyanin production, or disease, such as fungal growth... in a transformation in another 24 hours. The remaining starter culture can be used for colony PCR and...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules... mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids at Addgene...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... motor to auditory pathway important for vocal learning. Nat Neurosci 20(7), 978–986. PubMed PMID: 28504672...Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    ...your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic of the evolution...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies... secreted into the supernatant. Four plasmids containing different nanobodies (NB) were generated in pMAK... dual-deaminase base editor enables concurrent adenine and cytosine editing. Find the plasmids at Addgene...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016) C2c2 is a single-component programmable...Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017... L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional Characterization...Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (2015) Cpf1 Is a Single RNA-Guided ...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...are typically lysed in a non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X...nonspecific proteins from the sample prior to beginning the IP.  During the IP, the target protein in ...Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively, the protein can ...control, confirming that the capture antibody is functioning as expected. In order to identify any non-specific...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    ...managing a lot of references. I cite as I write, meaning I use the software to add the citations in real...write. Things are going to get a little crazy (meaning you are probably going to cite hundreds of references...better to keep your references organized from the beginning. I also recommend using the citation style of ...relevant to you, note it, and move on. At the beginning I felt like this was cheating. Eventually I learned...

  9. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...neurotropic variant of AAV9, is better than AAV9 at gaining access to the CNS of C57BL/6J mice following IV...6J mouse strains likely contributed to the unintentionally selection of a virus with mouse-strain dependent... to select novel AAV capsid variants could unintentionally limit the utility of candidate capsids.  Are...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  10. Lab to Office Culture Shock

    Type
    Blog Post
    ... feeling of camaraderie one gets from a well-functioning lab team. Obviously not everyone gets this feeling...environments outside of academia provide a formal training regime and the right set of tools to help you ...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and virtually every person... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

  11. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...truth is, there is a lot of software development happening at Addgene under the hood. A significant portion...moment a deposit kit is sent to a depositor, to streamlining the order fulfillment process to reduce viral...language and it took us a total of two years, from planning to completion, to do this upgrade while continuing...principles for open science. I hope you enjoyed learning about Addgene’s software development team and ...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally occurs in...especially if the infection is serious or life-threatening, treatment will often begin before this information...results. While this is not the only method of determining drug resistance status, it is a very common, ...essential component of preventing infection, as is cleaning and disinfecting frequently touched surfaces like...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...immortalized human cell line. He proved this by examining whether the cell lines had isoenzymes and genetic... piece of paper on the front of the incubator containing the passage number, cell line, and date, which...He studied biochemistry at Warwick University, gaining extensive cell culture research experience through...Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...of control plasmids Part of planning your experiment includes determining what factors need to be controlled...independent variable you are testing and not some other unintended factor. Control plasmids are used to minimize...systems have many variables. However daunting designing and executing these steps may be, proper controls...

  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually...Use BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...most experiments, the lac promoter can “leak,” meaning that a low level of expression exists even without...

  16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...might be considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep...the name suggests, these lentiviruses lack a functioning integrase, the enzyme responsible for inserting...homologous recombination or non-homologous end joining (see Figure 2).  Figure 2: Schematic representation...interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced by retroviral...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...backbone. This step requires “counter-selection,” meaning that the selection is for the loss of a specific...protein. This allows conjugation between a pir-containing donor and the recipient of interest. Because ...transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation of naturally...But if they are blue or magenta, they are real! Planning an allelic exchange experiment? Find the pTOX ...

  18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...protein. These were either (i) coded by the multiple cloning site and therefore consist of random amino acid... often use restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent...PVAT, when we use the AgeI site in the multiple cloning site) is sufficient. For the fluorescent protein-POI...immunofluorescence or properties of the protein.  Determining other aspects of functionality (catalytic activity...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... Chalk Talk These items should be part of the beginning of your chalk talk. These should be plainly stated... implicated in several human diseases. Proper patterning of cells and tissues is fundamental to development...you understand what it takes to be successful in running a lab, getting grants, and writing papers? This... and earn some cash for my expensive habits of running shoes and single malt whiskies, but I remember ...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... primarily by two pathways: Non-Homologous End-Joining (NHEJ) and Homology Directed Repair (HDR). NHEJ...your site of interest, and a donor DNA molecule containing your desired edit are all you need to generate...generate a knock-in.   General considerations for designing a repair template to create mutations HDR donors...Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve...
Showing: 501 - 520 of 985 results