Rabies and Neuronal Tracing
Type
Blog Post
...generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583–1601. doi: 10.1038/nprot.2013.094....
We narrowed to 666 results for: otos
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...spatiotemporally controllable version of the BioID protocol. The critical component for both BioID and Split-BioID... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...reproducibility. Non-recombinant antibodies are notorious for being the source of irreproducible data (Begley... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional... -
Of Myc and Men
TypeBlog Post... monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–3616. https... -
Degrading DNA with Cascade-Cas3
TypeBlog Post..., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...hours reviewing papers to piece together the best protocol; he wanted to get going and learn through doing... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...