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Showing: 501 - 520 of 654 results

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5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...page. A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...
Transferable Skills Guide: Managing a Team

Type

Blog Post

...hours reviewing papers to piece together the best protocol; he wanted to get going and learn through doing...
Of Myc and Men

Type

Blog Post

... monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–3616. https...
Degrading DNA with Cascade-Cas3

Type

Blog Post

..., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...
MXS Chaining

Type

Blog Post

... Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1. Anderson JC, Dueber JE, Leguia ...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

... the bench, this results in a shorter staining protocol because the primary antibody can be “labeled” ...
Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...-use AAV from Addgene here or check out these protocols if you want to package your own virus. ...

Showing: 501 - 520 of 654 results

Results per page:

5 Tips for Troubleshooting Viral Transductions

Hot Plasmids and Viral Preps - May 2021

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Fluorescent Proteins 101: Fluorescent Protein Timers

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Transferable Skills Guide: Managing a Team

Of Myc and Men

Degrading DNA with Cascade-Cas3

The AAVantages of AAV in CRISPR Screens

Fluorescent Tagging of Endogenous Genes with SapTrap

Plasmids 101: Knockout/Knock-In Plasmids

Plasmids 101: Optimizing Plasmid Yields

Anatomy of a Plasmid Page at Addgene

CRISPR 101: RNA Editing with Cas13

MXS Chaining

Plasmids 101: Secondary Nanobody Toolbox

Course-Based Undergraduate Research Experiences (CUREs)

AAVs CREATed for Gene Delivery to the CNS and PNS

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