We narrowed to 658 results for: otos

1. Rabies and Neuronal Tracing

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   Blog Post

   ...generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583–1601. doi: 10.1038/nprot.2013.094....

2. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   ...Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ...spatiotemporally controllable version of the BioID protocol. The critical component for both BioID and Split-BioID...

4. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative ...

5. 5 Tips for Troubleshooting Viral Transductions

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   ...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

6. Of Myc and Men

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   ... monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–3616. https...

7. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

10. Degrading DNA with Cascade-Cas3

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    ..., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo...

11. The AAVantages of AAV in CRISPR Screens

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    ...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...

12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

13. Transferable Skills Guide: Managing a Team

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    ...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...

14. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

16. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

17. Plasmids 101: Knockout/Knock-In Plasmids

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    ...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...

18. Plasmids 101: Optimizing Plasmid Yields

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    ...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...

19. Anatomy of a Plasmid Page at Addgene

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    ... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...

20. Course-Based Undergraduate Research Experiences (CUREs)

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    ...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),...