We narrowed to 660 results for: otos

...reproducibility. Non-recombinant antibodies are notorious for being the source of irreproducible data (Begley...

...knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...

...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...

...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...

...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...

...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

..., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo...

...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...

... monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–3616. https...

...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...

... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...

... the bench, this results in a shorter staining protocol because the primary antibody can be “labeled” ...

...-use AAV from Addgene here or check out these protocols if you want to package your own virus. ...

... Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1. Anderson JC, Dueber JE, Leguia ...