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We narrowed to 898 results for: plasmid

Showing: 501 - 520 of 898 results

  1. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...

  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... can clone the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...

  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  7. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...genetically encoded, and luckily, we have pre-designed plasmids for these systems! Ion sensors One common biosensor... vehicle, we’ve got you covered with packaging plasmids! References and Resources References  Bhalla ...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...typically express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate ...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...

  15. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

  17. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...for verifying plasmids like this is "plasmid fingerprinting", where you cut the plasmid into 3-8 pieces...used to verify your plasmid. The goal of a diagnostic digest is to cut your plasmid into specific sized...insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting" Often, it will be enough...for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more. Protocols...Digest You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction...selectively move a specific piece of DNA from one plasmid to another. A diagnostic restriction enzyme digest...called restrictions sites. Often, the size of the plasmid insert and vector backbone are known and thus this...

  18. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... 1μg of recipient plasmid. It is also critical that as much of the recipient plasmid as possible be cut...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

  19. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...isolate your plasmid DNA from the bacterial culture by following the Isolating Your Plasmid DNA protocol... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12–16 h on average. Certain features of a plasmid may render...Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture All Addgene...Culture Inoculating a Liquid Bacterial Culture Plasmids can carry one or more antibiotic resistance genes...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...
Showing: 501 - 520 of 898 results