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We narrowed to 759 results for: rest

Showing: 501 - 520 of 759 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...favorite Cas enzyme, a guide RNA to your site of interest, and a donor DNA molecule containing your desired... these numbers may vary based on your locus of interest and experimental system.  Synthesizing ssODNs ...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 copies of the short epitope...portion of the receptors recognizes an antigen of interest while the intracellular T cell receptor portion...

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...between a pir-containing donor and the recipient of interest. Because pDS132 cannot be replicated in the recipient...Depending on the growth rate of the organism of interest, from the day primers arrive, the desired chromosomal...

  4. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...trainees at low wages will still be there without restructuring. Positive Points for Trainees - Taking Steps...of Research Website Additional Publications of Interest Can Be Found Here Resources at Addgene  Read...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ... spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel...pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Watch the Video!...new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short stretch... Cloning (LIC) Scarless cloning with Type II restriction enzymes and T4 polymerase pLKO.1 - TRC Cloning...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either...Voltage Sensor  Ingaki and colleagues (8) were interested in using photoactivatable, optogenetic accuators...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...and repair pathways work downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert...Validated gRNAs to Target Your Genomic Locus of Interest Browse the CRISPR Collection ...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...novel base editor proteins. She is particularly interested in combining the realms of art and science. Follow...

  11. Western Blot

    Type
    Protocol
    ...protein of interest, and a negative control sample that does not express the protein of interest....detection using antibodies specific to the protein of interest. This protocol describes the basic steps for lysing... Precast SDS-PAGE gel SDS-PAGE running buffer Prestained protein ladder Ethanol iBlot 2 PVDF Mini Stack...depending on the cellular location of the protein of interest. RIPA buffer is suitable for most proteins but...the samples into the gel. Load 5–10 µL of the prestained protein ladder. Place the lid on the tank and...sample type and cellular location of the protein of interest. You may need to try a variety of lysis buffers...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... can inhibit important reactions like PCR or restriction digests. Transfection Grade DNA (high quality...than half 70% recycled materials Sustainable forestry certification Starch-based compostable bags for...

  14. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ... Annealed Oligo Cloning You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial ...this tutorial we will discuss how to add new restriction sites to the MCS of an empty vector. However,...design a set of oligos containing your desired restriction sites and add them to your existing vector. It...cloned directly into the overhangs generated by restriction digest of existing sites in the original vector...waiting for your oligos to arrive, conduct a restriction digest of 1μg of vector with EcoRI and SalI. ...

  15. Ligation Independent Cloning

    Type
    Protocol
    ...Empty vectors for LIC typically employ type II restriction enzymes (e.g. BsaI), which cut at a specified...overhangs with a single enzyme, and remove the restriction sites from the final product (no "cloning scars...suggested leader sequence fused to your gene of interest, in frame with the start codon or tag sequences...sequence and sacB counter-selection marker (see our restriction digest protocol ). After the digest is complete...corresponding to the first base removed by the restriction digest to create the free 5' end of the cut vector...

  16. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA Bacterial ...that have been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically...connecting the insert DNA (gene or fragment of interest) into a compatibly digested vector backbone. This...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic....A., and Callaway, E.M. (2007). Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  18. Video Library

    Type
    Protocol
    ...Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction Digest Protocol...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an Agarose...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials Material Vendor and...for plasmids that were successfully ligated by restriction enzyme digestion. However, once you have identified...use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with EcoRI and NcoI: 1 μg miniprep DNA...select for cells that stably express your shRNA of interest. E.1 Recommended Materials Material Vendor and...cells that are stably expressing your shRNA of interest. F.1. Recommended Materials Material Vendor and...

  20. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...DNA from an Agarose Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification...lanes between samples and between the ladder and nearest sample. To minimize the risk of DNA damage, it ...
Showing: 501 - 520 of 759 results