...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here... The sense of trust and comfort I had with my teammates acted as my safety net and trampoline all at once... I really believe that the culture at Addgene cultivates trust between coworkers and that trust then translated...

...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...opsonization of IgG and IgM antibodies on a pathogen activates the immune system's classical complement pathway.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...used in optogenetic studies are not native to vertebrates and are commonly introduced in zebrafish through... a neural circuit involved in locomotion that generates periodic motor commands for rhythmic movements...  Find plasmids from the Isacoff lab! They then tested what kind of swimming behavior, spontaneous swim...

...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression...bacterial cells and selectively grown on antibiotic plates.  Importantly, because the bacteria from which ...

...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky...time to completely digest the template DNA. Note—templates must have been isolated from methylation competent...

...breakthroughs often occur when the unexpected infiltrates the realm of focused exploration. Here, we delve...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...beneficial to carry a notebook at all times (or use the notes app on your phone) to capture fleeting thoughts ...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

... may appear to be binding at particular genomic sites when in fact they are just non-specific or indirectly... MNase. Once MNase has been localized to target sites, the nuclease is briefly activated to digest the... an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets,...

...carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in ...collection of empty backbones that have been designed, tested, and published by academic scientists. To help ...a mutated strain or a mutated version of YGOI to test the different phenotypes of the specific mutations...

... can’t safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase...I got good at this despite the fact that my lab mates thought this was hilarious.  Birth of the baby gel...

...labs have websites listing alumi and it is easy to use publication records, university websites, ResearchGate... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

...References Introduction The field of optogenetics integrates optics and genetic engineering to measure and...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude...msec (hChR2) to “long lasting,” e.g. seconds to minutes with stable step-function opsins (SSFOs). Opsin...flavin mononucleotide as its chromophore, and phosphorylates the transcriptional activator FixJ in the absence...

...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...in a competitive transplant assay in the mouse intestine, demonstrating its utility in long-term tracking...

...scicomm group project on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer...industry. Along this road, science communication graduates are often well-equipped. Many institutions are...explores risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications...

...have recently received a grant from the National Institutes of Health that will address this problem.The ...animals may be variable among animals and bleed dates. The development of monoclonal antibody (mAb) technology... represent the official views of the National Institutes of Health.     References and Resources References...

...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...be run in parallel and provide serotype data in minutes and the scripts  can be easily customized to detect...

...avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged ... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

...physical and chemical conditions like YNP thermal sites - are also of practical interest since they’ve previously...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...

...building that someone from a different company had tested positive for COVID-19 (it turned out to be an unfounded...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...

...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and transduction...host of other AAV serotypes; it will be exciting to test this approach with different capsids. Most importantly...