We narrowed to 625 results for: Emb

1. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

2. The time and cost required to make a plasmid

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   Blog Post

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

3. Transferable Skills Guide: Problem Solving

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   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

7. The Future of Research Symposium Boston 2015

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   Blog Post

   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

8. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

12. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

13. 6 Tips for Grant Writing

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    Blog Post

    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

14. Engaging with science and society at pgEd

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    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

15. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

17. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

19. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

20. CRISPR in the Clinic

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    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...