We narrowed to 772 results for: LIS

1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

   ...Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our Interview with Previous Awardee Kwabena ...

2. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

   ...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....

3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

   ... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the...

4. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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   Blog Post

   ...comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that converts...

5. Understanding GPCR Signaling Using TRUPATH

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   Blog Post

   ...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin...

6. Antibodies 101: Fc Effector Functions

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   Blog Post

   ...don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies...

7. Hot Plasmids - October 2022

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   Blog Post

   ...!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of...

8. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   Blog Post

   ... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation...

9. Plasmids 101: What is a plasmid?

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   Blog Post

   ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...

10. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post

    ...Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the next...

11. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    ...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading...

13. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post

    ...Addgene) don’t forget this in the future. Here’s a list of blog posts to help you tackle the challenges ...

14. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post

    ...what the user wants to do and make the path to accomplishing their goals as intuitive and painless as possible...

15. Using Addgene's New Viral Service for Your Research

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    Blog Post

    ... over to the Viral Service page to find the full list of what’s available or suggest additional plasmids...

16. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...

17. Plasmids 101: Origin of Replication

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    Blog Post

    ...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...

18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post

    ...Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 ...

19. Antibodies 101: Epitope Tags

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    Blog Post

    ... Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived from the protooncogene of the same name...

20. Antibodies 101: Chimeric Antibodies

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    Blog Post

    ...Protein B. The antibodies are both extensively published, knockout validated, and work in your application...