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Showing: 781 - 810 of 1014 results

  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...

  2. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...

  3. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...applications that don’t yet exist, but it wasn’t clear that this was even possible. There is a variety ...that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E. coli, in mammalian...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  13. MXS Chaining

    Type
    Blog Post
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  21. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  22. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

  23. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

  24. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

  25. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...

  26. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  27. Hot Plasmids - November 2023

    Type
    Blog Post
    ...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

  29. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for. The result is redundancy...organism. So what are the evolutionary constraints that lead to these preferences and what can we do about them...

  30. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...optimization within your experiment. Read on to learn more about the various controls that can be used...biological controls is independence: at the very least you should repeat your whole experiment from start...
Showing: 781 - 810 of 1014 results