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...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

...manipulate neural populations in order to get new insights into functional network interactions. Combining... bacterium that can sense contaminants and emit light as a response. To create this bacterial sensor, ...

... are new to you, we’ll go through examples to highlight the difference between them using a plasmid available...

...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

...you that Twitter is a tool worth using, but you might be wondering how to get started. This blog post ...video from Science Media Savvy At this point, you might be be thinking social media could be useful, but...

...researchers can use to characterize these variants in a high-throughput manner and therefore push us closer to...the function of rare cancer variants and genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations...

...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

...system that uses synthetic biology to target and light-up bacteria unwanted in food, healthcare settings...failure at all stages in the startup". To gain insight into "the random-walk" of a budding startup, I ...

...mentoring group Find 5-8 colleagues that you think might be interested and willing to commit to the group...same place, a 1-2 hour lunch on a regular schedule might work. If not, perhaps a 3 hour breakfast once a ...

...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

... is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated to the...interest. The optical density (OD) - the measure of light absorbed by a solution - of these wells is plotted...

...of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose it can be. Let... Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with NEB’s DAD Interview...

...Lambert-Eaton myasthenic syndrome (LEMS) because, at high concentrations (25 mM), Gdm+ binds to and inhibits...disease-linked Arg-mutant proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering...

...and BioID, any protein that’s within a ~10nm neighborhood of the bait protein will be biotinylated. This...Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local protein interactomes by proximity‐dependent...

...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...

...1948 as a variation of the more commonly observed light emission by fluorescence. The widespread use of ...distances that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources...