We narrowed to 628 results for: PROC

1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   ...for identifying genes involved in a biological process. These screens provide an alternative to genome-wide...

2. Hot Plasmids - November 2023

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   Blog Post

   ... more accessible by simplifying the production process and could even enable the creation of CAR-T cells...

3. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   Blog Post

   ...kanamycin resistance gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for...

4. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   ...editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The first deaminase-free...

5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...Culturing a scientific network We refer to the process of mentoring within a bin as Horizontal Transfer...

6. Mesothelioma - Causes, Symptoms, and Treatment

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   Blog Post

   ...mutate and ultimately form cancerous tumors.  This process is not quick. It can take decades from the first...

7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

8. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of...

9. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As mentioned...

10. Tips for Using FRET in Your Experiments

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    Blog Post

    ...defined FRET as "a distance-dependent physical process by which energy is transferred non-radiatively ...

11. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription, the promoter...

12. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

    ...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...identify two functionally distinct populations." Proceedings of the National Academy of Sciences 109.2 (2012...

15. Hot Plasmids: Summer 2024

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    Blog Post

    ... air-water interface (AWI) during the freezing process, resulting in sample loss. Ci Ji Lim’s lab found...

16. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    ...normalization is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could...

18. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion The...

19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...enables labeling of delicate axonal and dendritic processes not previously visible with Brainbow-1 and -2....

20. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid: the Escherichia...